| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Dual specificity protein kinase CLK1, CDC-like kinase 1, CLK1, CLK |
| Entrez GeneID | 1195 |
| WB Predicted band size | 57.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CLK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-99 amino acids from the N-terminal region of human CLK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CLK1 (N-term)抗体的3篇示例文献(内容为虚构示例，仅供参考)：
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1. **文献名称**: *"Characterization of CLK1 Kinase Activity in Spliceosome Assembly Using a Novel N-terminal Specific Antibody"*
**作者**: Zhang L, et al.
**摘要**: 本研究开发了一种针对CLK1蛋白N端表位的特异性抗体，验证了其在Western blot和免疫沉淀中的高亲和力。利用该抗体，作者发现CLK1通过磷酸化SR蛋白调控剪接体动态组装，并证实其在癌细胞中异常激活的机制。
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2. **文献名称**: *"CLK1 Inhibition Attenuates Neurodegeneration in a Drosophila Model of Alzheimer’s Disease"*
**作者**: Rodriguez S, et al.
**摘要**: 通过CLK1 (N-term)抗体的免疫荧光染色，作者在果蝇模型中观察到CLK1在神经细胞核内富集，并与tau蛋白异常磷酸化相关。抗体特异性验证显示其适用于体内外模型，为CLK1作为神经退行性疾病靶点提供证据。
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3. **文献名称**: *"Development and Validation of a CLK1-Selective Antibody for High-Content Screening in Drug Discovery"*
**作者**: Kim H, et al.
**摘要**: 本文报道了一种高特异性CLK1 N端抗体的开发，并应用于激酶抑制剂的高通量筛选。通过ELISA和免疫组化验证，该抗体能区分CLK1与其他CDC2样激酶家族成员(如CLK2/3)，显著提升靶向药物筛选效率。
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注：以上文献为模拟内容，实际引用需查询PubMed或SciHub等平台。若需真实文献，建议检索关键词“CLK1 antibody N-terminal”或抗体编号(如Abcam#12345)。
The CLK1 (N-term) antibody is a tool designed to target the N-terminal region of the CDC-like kinase 1 (CLK1) protein, a member of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. CLK1 plays a critical role in regulating pre-mRNA splicing by phosphorylating serine/arginine-rich (SR) proteins, which are essential for spliceosome assembly and alternative splicing. This kinase is involved in diverse cellular processes, including cell cycle progression, stress responses, and RNA metabolism, with implications in cancer, neurodegenerative diseases, and developmental disorders.
The CLK1 (N-term) antibody is commonly used in research to detect endogenous CLK1 expression via techniques like Western blotting, immunofluorescence, or immunoprecipitation. Its specificity for the N-terminal domain allows researchers to distinguish CLK1 from closely related family members (CLK2. CLK3. CLK4) and study its unique functional contributions. Studies leveraging this antibody have explored CLK1's role in splicing dysregulation linked to conditions such as Alzheimer's disease or its overexpression in certain cancers. Additionally, it aids in evaluating CLK1 inhibitors as potential therapeutics. Validation via knockout controls or siRNA-mediated knockdown is recommended to confirm specificity, as cross-reactivity may occur due to structural homology within the kinase family.
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