| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 5'-AMP-activated protein kinase subunit beta-2, AMPK subunit beta-2, PRKAB2 |
| Entrez GeneID | 5565 |
| WB Predicted band size | 30.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This AMPK beta2 (PRKAB2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-33 amino acids from the N-terminal region of human AMPK beta2 (PRKAB2). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于AMPK beta2 (PRKAB2) N端抗体的3篇参考文献，按研究领域和抗体应用场景整理：
1. **"AMPK β2 subunit is required for energy homeostasis during metabolic stress"**
*作者：Sakamoto K. 等人*
摘要：研究通过Western blot和免疫沉淀实验，使用抗PRKAB2 N端抗体，揭示了AMPK β2亚基在肝脏能量代谢中的关键作用，发现其缺失导致小鼠在高脂饮食下胰岛素敏感性显著下降。
2. **"Subcellular localization of PRKAB2 in breast cancer progression"**
*作者：Chen L. 团队*
摘要：利用针对PRKAB2 N端的单克隆抗体进行免疫荧光染色，发现乳腺癌细胞中β2亚基的胞质定位与肿瘤侵袭性相关，提示其可能作为转移性生物标志物。
3. **"Structural insights into AMPK complex assembly via β2 subunit-specific antibodies"**
*作者：Oakhill J.S. 小组*
摘要：通过N端特异性抗体阻断实验，结合冷冻电镜技术，阐明β2亚基的N端结构域在AMPK异源三聚体形成中的关键作用，为靶向药物设计提供依据。
*注：文献为示例性质，实际引用需根据具体研究补充DOI及期刊信息。*
The AMP-activated protein kinase (AMPK) beta2 subunit, encoded by the PRKAB2 gene, is a critical regulatory component of the heterotrimeric AMPK complex, which functions as a central energy sensor in eukaryotic cells. AMPK consists of a catalytic alpha subunit (α), a scaffolding beta subunit (β), and a regulatory gamma subunit (γ). The beta2 subunit (PRKAB2) plays a key role in stabilizing the complex, facilitating subcellular localization, and modulating AMPK activity in response to metabolic stress. The N-terminal region of the beta2 subunit contains a conserved domain critical for binding to the alpha and gamma subunits, enabling AMPK assembly and allosteric activation by AMP/ADP.
Antibodies targeting the N-terminal region of PRKAB2 (AMPK beta2) are widely used to study tissue-specific expression, post-translational modifications, and dynamic regulation of AMPK in metabolic pathways. These antibodies are essential tools for detecting beta2 in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Since AMPK beta2 is enriched in skeletal muscle, liver, and other metabolically active tissues, such antibodies help investigate its role in glucose uptake, lipid metabolism, and mitochondrial function. Dysregulation of AMPK beta2 has been linked to metabolic disorders, cancer, and neurodegenerative diseases, making these antibodies valuable for both basic research and translational studies. Specificity for the N-terminal region ensures minimal cross-reactivity with the closely related beta1 (PRKAB1) subunit, enabling precise analysis of beta2-dependent AMPK signaling.
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