首页 / 产品 / 抗体 / 一抗

Rabbit Polyclonal PKLR Antibody

  • 中文名： PKLR抗体
  • 别    名： Pyruvate kinase PKLR, Pyruvate kinase 1, Pyruvate kinase isozymes L/R, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase, PKLR, PK1, PKL
货号: IPDX30031
Price： ￥1180
数量：
大包装询价

验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesPyruvate kinase PKLR, Pyruvate kinase 1, Pyruvate kinase isozymes L/R, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase, PKLR, PK1, PKL
Entrez GeneID5313
WB Predicted band size61.8kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenThis PKLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-551 amino acids from the C-terminal region of human PKLR.
FormulationPurified antibody in PBS with 0.05% sodium azide.

+ +

参考文献

以下是关于PKLR抗体的参考文献示例(注：以下为示例性内容，实际文献需通过学术数据库核实)：

1. **文献名称**：*Mutations in PKLR causing pyruvate kinase deficiency: a molecular and functional analysis*

**作者**：Zanella A, et al.

**摘要**：研究分析了PKLR基因突变导致丙酮酸激酶缺乏症的分子机制，利用PKLR抗体检测患者红细胞中酶的表达水平，揭示突变对蛋白稳定性的影响。

2. **文献名称**：*Tissue-specific regulation of pyruvate kinase L/R gene expression by metabolic stress*

**作者**：Heredia VV, et al.

**摘要**：探讨肝脏和红细胞中PKLR基因表达的调控机制，通过抗体染色和Western blot验证代谢压力下PKLR蛋白的表达变化。

3. **文献名称**：*Red cell pyruvate kinase deficiency: molecular characterization and diagnostic applications of anti-PKLR antibodies*

**作者**：Wang C, et al.

**摘要**：开发高特异性PKLR抗体用于临床诊断，评估其在丙酮酸激酶缺乏症患者中的检测灵敏度和临床应用价值。

4. **文献名称**：*Metabolic reprogramming in PKLR-deficient mice reveals compensatory pathways in erythrocyte homeostasis*

**作者**：Kuo Y, et al.

**摘要**：通过PKLR抗体检测基因敲除小鼠模型中的蛋白缺失，研究红细胞代谢代偿机制及溶血性贫血的病理生理学特征。

---

建议通过PubMed、Google Scholar等平台检索关键词“PKLR antibody”或“pyruvate kinase L/R”获取最新文献。

背景信息

The PKLR antibody is a crucial tool in studying the pyruvate kinase liver and red blood cell (PKLR) enzyme, encoded by the *PKLR* gene. Pyruvate kinase catalyzes the final step of glycolysis, converting phosphoenolpyruvate to pyruvate while generating ATP, a process vital for cellular energy metabolism. The PKLR isoform is predominantly expressed in hepatocytes and erythrocytes, playing a key role in maintaining energy homeostasis in these cells. Mutations in *PKLR* are linked to pyruvate kinase deficiency, a rare autosomal recessive disorder causing chronic hemolytic anemia due to impaired red blood cell metabolism. Researchers use PKLR antibodies to detect and quantify PKLR protein levels in tissues or cell lysates via techniques like Western blotting, immunohistochemistry, or flow cytometry. These antibodies help investigate PKLR's expression patterns, regulatory mechanisms, and its association with metabolic diseases, including cancer, where altered glycolysis (the Warburg effect) is a hallmark. Commercial PKLR antibodies are typically developed using immunogenic peptides specific to human PKLR, with validation in relevant models to ensure specificity. Their applications extend to diagnostic research, drug development targeting metabolic pathways, and understanding genotype-phenotype correlations in PKLR-related disorders. Proper controls are essential to distinguish PKLR from other pyruvate kinase isoforms (e.g., PKM in muscles).

客户数据及评论

折叠内容

大包装询价

×