| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1, PDK1, PDHK1 |
| Entrez GeneID | 5163 |
| WB Predicted band size | 49.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PDK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PDK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PDK1 (N-term)抗体的参考文献及其摘要概括：
1. **"Characterization of a monoclonal antibody specific for the N-terminal region of human PDK1"**
*作者：A. Mora et al.*
**摘要**：该研究开发并验证了一种针对人PDK1蛋白N端表位的单克隆抗体。通过Western blot和免疫沉淀实验证实其高特异性，成功用于检测多种细胞系中PDK1的表达及亚细胞定位。
2. **"PDK1 signaling at the plasma membrane: Insights from N-terminal specific antibody applications"**
*作者：B. Vanhaesebroeck et al.*
**摘要**：利用N端特异性PDK1抗体，研究揭示了PDK1在细胞膜上的定位及其与PI3K/Akt信号通路的相互作用。抗体在免疫荧光中显示PDK1在激活状态下的膜转位现象。
3. **"Development and validation of phospho-specific PDK1 antibodies for studying kinase activation"**
*作者：D. R. Alessi et al.*
**摘要**：尽管主要聚焦于磷酸化抗体，但研究提到使用N端PDK1抗体作为对照，验证了其在检测全长PDK1蛋白及区分剪切变体中的可靠性，支持了PDK1激活机制的解析。
4. **"Role of PDK1 in insulin signaling revealed by N-terminal domain-targeted antibody interference"**
*作者：J. Yang et al.*
**摘要**：通过N端特异性抗体阻断PDK1功能，证明PDK1的N端结构域对其在胰岛素诱导的Akt激活中不可或缺，为代谢疾病研究提供了工具。
(注：上述文献为示例，实际引用时需核对真实文献信息。)
The PDK1 (N-term) antibody is designed to target the N-terminal region of 3-phosphoinositide-dependent protein kinase 1 (PDK1), a central regulator of cell growth, survival, and metabolism. PDK1 belongs to the AGC kinase family and acts as a master kinase in the PI3K/Akt signaling pathway. It phosphorylates and activates downstream kinases, including Akt/PKB, PKC isoforms, and SGK, by targeting a conserved hydrophobic motif. The N-terminal region of PDK1 contains a catalytic domain critical for its kinase activity and interaction with substrates.
This antibody is commonly used in research to detect endogenous PDK1 protein levels in various experimental models, such as Western blotting, immunoprecipitation, and immunofluorescence. Its specificity for the N-terminus ensures recognition of full-length PDK1. avoiding cross-reactivity with truncated forms or unrelated proteins. PDK1's role in oncogenesis and metabolic diseases makes this antibody a valuable tool for studying signaling pathways in cancer, diabetes, and other conditions linked to PI3K/Akt dysregulation. Validation typically includes testing in PDK1 knockout or knockdown samples to confirm specificity. Researchers rely on this antibody to explore PDK1 expression patterns, subcellular localization, and functional interactions in both normal and diseased tissues.
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