| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mitogen-activated protein kinase 10, MAP kinase 10, MAPK 10, MAP kinase p49 3F12, Stress-activated protein kinase 1b, SAPK1b, Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, MAPK10, JNK3, JNK3A, PRKM10, SAPK1B |
| Entrez GeneID | 5602 |
| WB Predicted band size | 52.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This MAPK10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MAPK10. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于MAPK10 (N-term)抗体的3篇参考文献，包含文献名称、作者及简要摘要内容：
1. **文献名称**: *"JNK3 Signaling in the Nervous System: Implications for Neuronal Survival and Degeneration"*
**作者**: Davis RJ, et al.
**摘要**: 该研究利用MAPK10 (N-term)抗体通过免疫印迹分析JNK3在小鼠脑组织中的表达模式，揭示了其在神经元应激反应中的激活机制，并探讨其在神经退行性疾病中的潜在作用。
2. **文献名称**: *"Selective Interaction of MAPK10 with Scaffold Proteins in Stress-activated Pathways"*
**作者**: Whitmarsh AJ, et al.
**摘要**: 通过免疫共沉淀(Co-IP)结合MAPK10 (N-term)抗体，研究发现了JNK3与特定支架蛋白的相互作用，阐明了其在MAPK信号通路中的选择性调控机制。
3. **文献名称**: *"Role of JNK3 in Beta-Amyloid-Induced Neuronal Apoptosis"*
**作者**: Morishima Y, et al.
**摘要**: 该文献使用MAPK10 (N-term)抗体进行免疫组化实验，证实了JNK3在β-淀粉样蛋白诱导的神经元凋亡中的关键作用，为阿尔茨海默病治疗提供了新靶点。
4. **文献名称**: *"Antibody Validation for Specific Detection of MAPK10 Isoforms in Human Tissues"*
**作者**: Kumar S, et al.
**摘要**: 研究系统验证了MAPK10 (N-term)抗体的特异性，通过敲除细胞模型和质谱分析，确认其能选择性识别JNK3的N端区域，适用于人脑组织样本的蛋白定位研究。
(注：以上文献信息为模拟示例，实际引用需根据具体论文调整。)
The MAPK10 (N-term) antibody is designed to target the N-terminal region of mitogen-activated protein kinase 10 (MAPK10), also known as c-Jun N-terminal kinase 3 (JNK3). MAPK10 is a member of the serine/threonine protein kinase family, which plays a critical role in stress-response signaling pathways, regulating cellular processes such as apoptosis, differentiation, and proliferation. Unlike JNK1 (MAPK8) and JNK2 (MAPK9), JNK3 is predominantly expressed in the brain, heart, and testes, and is closely associated with neurodegenerative disorders, including Alzheimer’s and Parkinson’s diseases, due to its involvement in neuronal apoptosis.
The N-terminal region of MAPK10 is essential for kinase activation and interaction with upstream regulators (e.g., MKK4/7) and downstream substrates. Antibodies targeting this region are widely used to detect endogenous MAPK10 expression levels, monitor its activation status, or study its subcellular localization in experimental models. These antibodies are typically validated for applications such as Western blotting, immunohistochemistry, and immunoprecipitation. Specificity is ensured by epitope alignment and cross-reactivity tests against other MAPK isoforms.
Research utilizing MAPK10 (N-term) antibodies has advanced understanding of JNK3's role in stress-induced signaling, neuroinflammation, and cancer progression. Dysregulation of MAPK10 has been linked to tumor suppression, neuronal survival, and responses to oxidative stress, making it a potential therapeutic target. The antibody’s ability to distinguish MAPK10 from other JNK isoforms supports precise mechanistic studies in both physiological and pathological contexts.
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