| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase Sgk3, Cytokine-independent survival kinase, Serum/glucocorticoid-regulated kinase 3, Serum/glucocorticoid-regulated kinase-like, SGK3, CISK, SGKL |
| Entrez GeneID | 100533105;23678 |
| WB Predicted band size | 57.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SGK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SGK3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于SGK3 (N-term)抗体的模拟参考文献示例，基于相关领域的研究方向概括而成：
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1. **文献名称**：*"SGK3 Phosphorylation and Its Role in PI3K Pathway Regulation"*
**作者**：Zhang, Y., et al. (2019)
**摘要**：本研究利用SGK3 (N-term)抗体通过Western blot和免疫沉淀技术，揭示了SGK3在PI3K信号通路中的磷酸化调控机制，发现其N端结构域对激酶活性的稳定性和亚细胞定位至关重要。
2. **文献名称**：*"SGK3 Expression in Triple-Negative Breast Cancer: A Prognostic Marker"*
**作者**：Lee, H., & Kim, S. (2020)
**摘要**：通过免疫组化实验结合SGK3 (N-term)抗体，作者证实SGK3在乳腺癌组织中的高表达与患者不良预后相关，并提示其可能通过调控EMT过程促进肿瘤转移。
3. **文献名称**：*"SGK3 N-Terminal Antibody Validation for Functional Studies in Neuronal Cells"*
**作者**：Müller, R., et al. (2018)
**摘要**：该研究系统验证了SGK3 (N-term)抗体的特异性，并应用于神经元细胞的免疫荧光实验，证明SGK3在氧化应激条件下通过mTORC2通路调控细胞存活。
4. **文献名称**：*"SGK3 Deficiency Alters Autophagy in Mouse Models"*
**作者**：Chen, J., et al. (2021)
**摘要**：利用SGK3 (N-term)抗体检测基因敲除小鼠模型中的蛋白表达缺失，发现SGK3通过调节自噬相关蛋白LC3-II的生成影响代谢稳态。
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**注**：以上文献为基于SGK3相关研究的合理模拟，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。若需具体文章，建议使用关键词“SGK3 antibody N-terminal”或“SGK3 kinase domain”进一步查找。
The SGK3 (N-term) antibody is designed to target the N-terminal region of serum- and glucocorticoid-regulated kinase 3 (SGK3), a serine/threonine protein kinase involved in diverse cellular processes. SGK3. part of the SGK family alongside SGK1 and SGK2. is regulated by phosphoinositide 3-kinase (PI3K) signaling and shares structural homology with Akt kinases. It plays roles in cell survival, proliferation, and ion transport, often acting downstream of growth factors, hormones, or cellular stress. SGK3 is distinct in its phosphatidylinositol 3.4-bisphosphate (PI(3.4)P2)-dependent activation and localization to endosomal compartments, influencing pathways like mTORC1 and Wnt/β-catenin signaling.
The antibody’s specificity for the N-terminal region allows detection of endogenous SGK3 in applications such as Western blotting, immunoprecipitation, and immunohistochemistry. This region contains unique epitopes critical for distinguishing SGK3 from closely related kinases (e.g., SGK1/SGK2), minimizing cross-reactivity. SGK3 has been implicated in cancer progression (e.g., breast, ovarian), metabolic disorders, and neurodegenerative diseases, with studies highlighting its role in drug resistance and cellular adaptation to nutrient deprivation. Researchers utilize this antibody to explore SGK3’s expression patterns, post-translational modifications, and interactions in disease models, aiding mechanistic insights and therapeutic targeting. Validation often includes knockout controls to confirm specificity.
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