| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis, BBC3, PUMA |
| Entrez GeneID | 27113 |
| WB Predicted band size | 20.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于Puma BH3结构域抗体的3篇参考文献示例，包括文献名称、作者及摘要内容概括：
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1. **文献名称**：*"Development and Characterization of a Monoclonal Antibody Specific for the Pro-Apoptotic PUMA BH3 Domain"*
**作者**：Chen L et al.
**摘要**：该研究报道了一种针对PUMA蛋白BH3结构域的单克隆抗体的开发与验证。通过免疫印迹和免疫荧光实验，证实该抗体能特异性识别PUMA的BH3结构域，并成功用于检测细胞内PUMA的激活及与抗凋亡蛋白Bcl-2的相互作用，为研究PUMA依赖的凋亡通路提供了工具。
2. **文献名称**：*"PUMA BH3 Domain-Mediated Apoptosis in Cancer Cells: A Functional Study Using Domain-Specific Antibodies"*
**作者**：Wang X et al.
**摘要**：本研究利用针对PUMA BH3结构域的抗体，探究其在癌细胞凋亡中的作用。实验表明，阻断PUMA BH3结构域可抑制化疗药物诱导的线粒体凋亡通路，并通过共聚焦显微镜观察到抗体对PUMA与Bcl-xL结合的竞争性抑制，揭示了BH3结构域在凋亡信号中的关键功能。
3. **文献名称**：*"A Novel Anti-PUMA BH3 Antibody Enhances Chemosensitivity in Colorectal Carcinoma Models"*
**作者**：Kimura S et al.
**摘要**：该文献描述了一种新型抗PUMA BH3抗体在结直肠癌模型中的应用。研究发现，该抗体通过靶向PUMA的BH3结构域，增强肿瘤细胞对5-氟尿嘧啶的敏感性，并证实其通过上调caspase-3活性促进凋亡，为联合治疗策略提供了实验依据。
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*注：上述文献为示例性内容，实际研究中请根据具体发表的论文调整信息。建议通过PubMed或Web of Science以关键词“PUMA BH3 domain antibody”检索最新文献。*
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