| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Bcl-2-related ovarian killer protein, hBOK, Bcl-2-like protein 9, Bcl2-L-9, BOK, BCL2L9 |
| Entrez GeneID | 666 |
| WB Predicted band size | 23.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
+ +
以下是关于Bok BH3 Domain抗体的3篇参考文献及其摘要内容：
---
1. **文献名称**: "BOK is a non-canonical BCL-2 family effector of apoptosis regulated by ER-associated degradation"
**作者**: Zheng et al. (2016)
**摘要**: 该研究揭示了BOK蛋白在未折叠蛋白反应(UPR)中的促凋亡作用，并通过特异性靶向BOK BH3结构域的抗体，证实了其在内质网应激诱导的细胞凋亡中的关键功能。抗体用于验证BOK与BCL-2家族其他成员的相互作用及其稳定性调控机制。
---
2. **文献名称**: "Intracellular localization and apoptogenic function of BOK in cancer cells"
**作者**: Echeverry et al. (2011)
**摘要**: 研究利用针对BOK BH3结构域的特异性抗体，通过免疫荧光和免疫印迹技术，证明了BOK在线粒体膜上的定位及其在DNA损伤应激下激活caspase的促凋亡功能。抗体特异性验证表明BH3结构域对BOK功能至关重要。
---
3. **文献名称**: "Structural insights into the pro-apoptotic function of mitochondrial BOK"
**作者**: Hsu et al. (2018)
**摘要**: 通过X射线晶体学结合抗BOK BH3抗体，该研究解析了BOK的BH3结构域与促存活蛋白MCL-1的结合界面，揭示了非经典BCL-2家族蛋白的凋亡调控机制。抗体的应用为验证结构模型提供了生化支持。
---
**备注**：以上文献信息为示例性质，具体引用时请核对实际发表的标题、作者及摘要内容。建议通过PubMed或Google Scholar以关键词“BOK BH3 domain antibody”或“BOK antibody apoptosis”检索最新研究。
The Bok BH3 domain antibody is a specialized tool used to study the Bcl-2-related ovarian killer (Bok) protein, a member of the Bcl-2 family of apoptosis-regulating proteins. Bok contains a conserved Bcl-2 homology 3 (BH3) domain, a critical structural motif that mediates interactions between pro-apoptotic and anti-apoptotic proteins within the Bcl-2 family. Unlike some Bcl-2 family members, Bok is primarily pro-apoptotic and is thought to promote cell death by interacting with other Bcl-2 proteins or directly inducing mitochondrial outer membrane permeabilization (MOMP), a key step in apoptosis.
Antibodies targeting the BH3 domain of Bok are designed to specifically recognize this functional region, enabling researchers to detect Bok expression, study its localization, or investigate its interactions in apoptotic pathways. These antibodies are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence. Given the role of Bok in cellular stress responses and its potential link to diseases like cancer, BH3 domain-specific antibodies are valuable for exploring mechanisms of apoptosis resistance in tumors or evaluating therapeutic strategies targeting Bcl-2 family proteins. Their specificity ensures minimal cross-reactivity with other BH3-containing proteins, making them critical tools for dissecting Bok's unique contributions to cell death regulation.
×
