| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AF4/FMR2 family member 3, Lymphoid nuclear protein related to AF4, Protein LAF-4, AFF3, LAF4 |
| Entrez GeneID | 3899 |
| WB Predicted band size | 133.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This LAF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1197-1227 amino acids from the C-terminal region of human LAF4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LAF4抗体的示例参考文献(示例为虚构，仅用于格式展示)：
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1. **文献名称**: *LAF4 Antibody Characterization in Acute Lymphoblastic Leukemia*
**作者**: Smith J, et al.
**摘要**: 该研究开发了一种高特异性LAF4单克隆抗体，并通过Western blot和免疫组化验证其在检测MLL-AF4融合蛋白中的有效性，为白血病分子分型提供新工具。
2. **文献名称**: *Role of LAF4 in Transcriptional Regulation: Insights from Antibody-Based Knockdown*
**作者**: Chen L, et al.
**摘要**: 利用LAF4抗体进行染色质免疫沉淀(ChIP)，揭示了LAF4蛋白在调控造血干细胞关键基因(如HOXA9)转录中的直接作用，并探讨其与白血病发生的关系。
3. **文献名称**: *Development of a Polyclonal LAF4 Antibody for Protein Interaction Studies*
**作者**: García R, et al.
**摘要**: 报道了一种兔源多克隆LAF4抗体的制备与验证，成功应用于免疫共沉淀(Co-IP)实验，识别出LAF4与RNA聚合酶II的相互作用，提示其在转录延伸中的功能。
4. **文献名称**: *LAF4 Expression Dynamics During B-Cell Differentiation*
**作者**: Tanaka K, et al.
**摘要**: 通过LAF4抗体进行流式细胞术和免疫荧光分析，发现LAF4蛋白在B细胞早期分化阶段高表达，可能参与淋巴细胞发育异常的病理机制。
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注：以上文献为示例，实际研究中请通过学术数据库(如PubMed)检索真实文献。
LAF4 (Lymphoid Nuclear Protein Related to AF4), also known as AFF3. is a member of the AF4/FMR2 family of nuclear transcriptional regulators. It plays a critical role in transcriptional elongation and chromatin modification, particularly during lymphoid development. The LAF4 protein is structurally characterized by conserved homology regions (AF4/FMR2 family domains) that mediate interactions with other transcriptional regulators, including components of the super elongation complex (SEC). Dysregulation of LAF4 is notably implicated in oncogenesis, especially in T-cell acute lymphoblastic leukemia (T-ALL), where chromosomal translocations involving the AFF3/LAF4 gene (e.g., t(2;11)(q31;q23)) generate fusion proteins that aberrantly activate oncogenic signaling pathways, such as those driven by NOTCH1.
LAF4 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation to investigate LAF4’s role in normal hematopoiesis and leukemogenesis. Commercially available antibodies often target specific epitopes within the N-terminal or C-terminal regions, enabling researchers to distinguish wild-type LAF4 from fusion variants. Validated LAF4 antibodies have contributed to identifying its involvement in transcriptional dysregulation, chromatin remodeling, and cross-talk with signaling pathways like Wnt/β-catenin. Ongoing research continues to explore LAF4’s potential as a diagnostic marker or therapeutic target in lymphoid malignancies.
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