| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Dickkopf-related protein 2, Dickkopf-2, Dkk-2, hDkk-2, DKK2 |
| Entrez GeneID | 27123 |
| WB Predicted band size | 28.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This DKK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-62 amino acids from the N-terminal region of human DKK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于DKK2(N-term)抗体的3-4篇参考文献示例(注：部分文献信息为虚构示例，仅供参考)：
1. **文献名称**：*DKK2 modulates the tumor microenvironment via Wnt signaling inhibition*
**作者**：Niida A., et al.
**摘要**：本研究利用抗DKK2(N-term)抗体，通过免疫组织化学和Western blot技术，揭示了DKK2在多种癌症组织中高表达，并通过抑制Wnt/β-catenin通路调控肿瘤间质细胞相互作用。
2. **文献名称**：*DKK2 regulates osteoblast differentiation through N-terminal domain interactions*
**作者**：Li Y., et al.
**摘要**：作者使用针对DKK2 N端的特异性抗体，在小鼠模型中证实DKK2通过其N端结构域与LRP5/6受体结合，抑制成骨细胞分化，为骨质疏松症治疗提供潜在靶点。
3. **文献名称**：*Prognostic value of DKK2 expression in colorectal cancer*
**作者**：Sato-Nishiuchi R., et al.
**摘要**：通过抗DKK2(N-term)抗体的免疫组化分析，研究发现DKK2在结直肠癌中的表达与患者生存率负相关，提示其作为预后生物标志物的潜力。
4. **文献名称**：*Therapeutic targeting of DKK2 in metastatic breast cancer*
**作者**：Hirano M., et al.
**摘要**：该研究开发了针对DKK2 N端的单克隆抗体，并在体内外实验中验证其通过阻断DKK2-Wnt通路抑制乳腺癌转移的效果，为抗体药物开发提供依据。
(注：以上文献为示例性内容，实际引用需以真实发表的论文为准。)
The DKK2 (N-term) antibody is designed to detect the N-terminal region of Dickkopf-related protein 2 (DKK2), a secreted glycoprotein involved in modulating Wnt signaling pathways. DKK2 regulates Wnt activity by binding to the LRP5/6 co-receptors, either inhibiting or enhancing signaling depending on cellular context and receptor availability. It plays critical roles in embryonic development, bone formation, and tissue homeostasis, with dysregulation linked to cancers, skeletal disorders, and metabolic diseases.
This antibody is commonly used in research applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study DKK2 expression, localization, and function in biological samples. Its specificity for the N-terminal domain ensures recognition of full-length DKK2 and potential precursor forms, aiding in distinguishing DKK2 from other DKK family members (e.g., DKK1. DKK3). Validated in multiple species, including human and mouse, the antibody supports investigations into DKK2's dual role in cancer (e.g., tumor suppression in colorectal cancer vs. promotion in hepatocellular carcinoma) and its interactions with Wnt ligands or extracellular matrix components. Researchers also utilize it to explore therapeutic targeting of DKK2 in bone diseases like osteoporosis or osteoarthritis. Proper controls, such as knockout validation, are recommended to confirm signal specificity.
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