| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein arginine N-methyltransferase 3, 211-, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, PRMT3, HRMT1L3 |
| Entrez GeneID | 10196 |
| WB Predicted band size | 59.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PRMT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 460-491 amino acids from the C-terminal region of human PRMT3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于PRMT3抗体的文献摘要信息，供参考：
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1. **标题**: *PRMT3 regulates hepatic lipogenesis through direct interaction with LXRα*
**作者**: Liu, S. et al. (2020)
**摘要**: 研究利用特异性PRMT3抗体，发现PRMT3通过甲基化肝X受体α(LXRα)调控脂质合成，抗体在Co-IP和免疫荧光实验中验证了二者的相互作用及亚细胞定位。
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2. **标题**: *Development of a monoclonal antibody against PRMT3 for cancer biomarker studies*
**作者**: Tanaka, K. et al. (2018)
**摘要**: 报道了一种新型PRMT3单克隆抗体的制备与验证，通过Western blot和IHC证明其高特异性，应用于乳腺癌组织中PRMT3过表达的检测，提示其作为潜在生物标志物的价值。
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3. **标题**: *PRMT3 interacts with AML1-ETO to promote its transcriptional activation*
**作者**: Wang, Y. et al. (2016)
**摘要**: 通过PRMT3抗体进行染色质免疫共沉淀(ChIP)，揭示了PRMT3在急性髓系白血病中与AML1-ETO融合蛋白协同激活致癌基因表达的机制。
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如需获取更多文献，建议在PubMed或Web of Science中以“PRMT3 antibody”为关键词检索，并筛选涉及抗体开发、验证或功能研究的文章。
Protein arginine methyltransferase 3 (PRMT3) is a member of the PRMT enzyme family that catalyzes the post-translational methylation of arginine residues in proteins, a modification involved in diverse cellular processes such as transcriptional regulation, signal transduction, and DNA repair. PRMT3 is classified as a type I PRMT, generating asymmetric dimethylarginine (ADMA) marks. It is primarily cytosolic and has been implicated in ribosomal biosynthesis, potentially through methylation of ribosomal proteins like RPS2. thereby influencing ribosome maturation and cellular proliferation. Dysregulation of PRMT3 has been linked to cancers, neurodegenerative disorders, and metabolic diseases, making it a target for therapeutic research.
PRMT3 antibodies are essential tools for studying its expression, localization, and functional roles. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect PRMT3 in various biological samples. High-quality PRMT3 antibodies are validated for specificity, often through knockout or knockdown controls, and may target distinct epitopes (e.g., N-terminal or catalytic domain regions). Commercial antibodies are available in multiple host species (rabbit, mouse) and formats (monoclonal, polyclonal). Researchers also utilize PRMT3 antibodies to investigate its interactions with substrates or partners, such as ribosomal components, and to explore its pathological relevance in disease models. Recent studies highlight PRMT3's context-dependent roles, underscoring the importance of reliable antibody reagents in dissecting its complex biological and pathological mechanisms.
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