| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII |
| Entrez GeneID | 4846 |
| WB Predicted band size | Calculated MW: 133 kDa; Observed MW: 140 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human eNOS. AA range:1144-1193 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3条与eNOS抗体相关研究的简要参考文献示例(内容基于学术文献的典型结构概括，非真实文献)：
1. **文献名称**：*"Endothelial nitric oxide synthase expression in atherosclerosis: A comparative study of antibody specificity"*
**作者**：Smith A, et al.
**摘要**：比较了不同商业eNOS抗体在动脉粥样硬化组织中的特异性，发现部分抗体存在交叉反应，强调验证抗体特异性对研究eNOS蛋白表达的重要性。
2. **文献名称**：*"Role of eNOS in vascular endothelial function: Insights from knockout mice models using immunohistochemistry"*
**作者**：Zhang L, et al.
**摘要**：通过eNOS基因敲除小鼠模型，结合特异性抗体进行免疫组化分析，证实eNOS缺失导致内皮依赖性血管舒张功能受损，抗体定位结果支持eNOS在内皮细胞中的关键作用。
3. **文献名称**：*"Altered eNOS phosphorylation status in diabetic rats detected by phospho-specific antibodies"*
**作者**：Brown K, et al.
**摘要**：利用磷酸化eNOS(Ser1177)特异性抗体，发现糖尿病大鼠模型中eNOS活性位点磷酸化水平显著降低，提示其与血管功能障碍的关联。
(注：以上为示例性概括，实际文献需通过PubMed/Google Scholar等平台检索确认。)
Endothelial nitric oxide synthase (eNOS) antibodies are essential tools in studying the role of eNOS, an enzyme critical for nitric oxide (NO) production in vascular homeostasis. eNOS, encoded by the *NOS3* gene, catalyzes L-arginine conversion to NO, a key signaling molecule regulating vasodilation, blood flow, and vascular remodeling. Dysregulated eNOS activity is linked to cardiovascular diseases (e.g., hypertension, atherosclerosis) and metabolic disorders, making its detection vital for understanding disease mechanisms.
eNOS antibodies target specific epitopes of the enzyme, enabling researchers to quantify protein levels, assess post-translational modifications (e.g., phosphorylation at Ser1177 or Thr495), and localize eNOS within tissues or cells via techniques like Western blotting, immunohistochemistry, or immunofluorescence. Some antibodies distinguish between inactive (monomeric) and active (dimerized) eNOS forms, providing insights into enzyme activation.
These antibodies are also used to study eNOS interactions with regulatory proteins, such as caveolin-1 or Hsp90. and its subcellular distribution in endothelial cells. Validated eNOS antibodies are rigorously tested for specificity using knockout controls, ensuring reliable results. Their applications span basic research, drug development, and clinical studies aimed at modulating NO signaling pathways. By elucidating eNOS dysfunction, these reagents contribute to therapeutic strategies for vascular and inflammatory diseases.
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