| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; MAPK1; ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK. |
| Entrez GeneID | 5595/5594 |
| WB Predicted band size | Calculated MW: 42,44 kDa; Observed MW: 42,44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p44/42 MAPK. AA range:330-379 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于ERK1/2抗体的3篇参考文献示例：
1. **文献名称**：*ERK1/2 MAP kinases: structure, function, and regulation*
**作者**：Roskoski, R.
**摘要**：该综述详细阐述了ERK1/2的结构、功能及其在信号转导中的调控机制，提及多种ERK1/2抗体的应用(如Western Blot和免疫组化)，并强调抗体特异性验证的重要性(如通过敲除细胞系验证)。
2. **文献名称**：*A rapid and sensitive method for detection of phosphorylated ERK1/2 by western blot*
**作者**：Ahn, N.G. et al.
**摘要**：研究提出了一种优化检测磷酸化ERK1/2(Thr202/Tyr204)的Western Blot方法，使用CST公司的#9102抗体，验证了其在细胞应激反应(如EGF刺激)中的高灵敏度和特异性。
3. **文献名称**：*Targeting ERK1/2 in cancer therapy: roles of pharmacodynamic biomarkers*
**作者**：McKay, M.M. et al.
**摘要**：本文探讨ERK1/2在癌症中的激活机制及靶向治疗策略，通过多种ERK1/2抗体(如总ERK和磷酸化抗体)分析肿瘤模型中的信号动态，验证抗体在临床前研究中的可靠性。
(注：以上文献信息为示例，实际引用需根据具体研究调整作者、标题和年份。)
**Background of ERK1/2 Antibodies**
Extracellular signal-regulated kinases 1 and 2 (ERK1/2), also known as MAPK3/1. are serine/threonine kinases central to the Ras-Raf-MEK-ERK signaling cascade. This pathway regulates diverse cellular processes, including proliferation, differentiation, survival, and apoptosis. ERK1 (44 kDa) and ERK2 (42 kDa) share ~85% amino acid sequence homology and are activated via dual phosphorylation at conserved threonine and tyrosine residues (Thr202/Tyr204 in ERK1; Thr185/Tyr187 in ERK2) by upstream MEK1/2 kinases. Dysregulation of ERK1/2 signaling is implicated in cancer, inflammation, and neurodegenerative diseases, making these proteins critical targets for research and therapeutic development.
ERK1/2 antibodies are essential tools for detecting expression, activation, and localization of these kinases in cells and tissues. They are commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Phospho-specific ERK1/2 antibodies distinguish the active, phosphorylated forms, enabling studies on pathway activation under experimental conditions (e.g., growth factor stimulation or drug treatment). "Total" ERK1/2 antibodies recognize both phosphorylated and unphosphorylated isoforms, serving as loading controls. These antibodies are often generated in rabbits or mice using synthetic peptides corresponding to conserved regions or phosphorylation sites.
Validation of ERK1/2 antibodies includes testing for cross-reactivity with other MAPK family members and verifying specificity via knockout controls. Their applications span basic research, drug discovery, and clinical diagnostics, particularly in oncology, where ERK1/2 hyperactivation is a hallmark of many cancers.
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