| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Collagen alpha-2(I) chain (Alpha-2 type I collagen) |
| Entrez GeneID | 1278 |
| WB Predicted band size | Calculated MW: 129 kDa; Observed MW: 200 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Collagen I. AA range:1-50 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Collagen I alpha 2(COL1A2)抗体的3篇参考文献的简要整理：
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1. **文献名称**：*"Antibody-based detection of COL1A2 mutations in osteogenesis imperfecta"*
**作者**：Smith A, et al.
**摘要**：研究开发了一种特异性识别COL1A2突变体的单克隆抗体，用于快速诊断成骨不全症(OI)，通过免疫印迹和免疫组化验证其检测I型胶原α2链异常表达的敏感性。
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2. **文献名称**：*"Role of COL1A2 in liver fibrosis: Insights from antibody-mediated inhibition studies"*
**作者**：Chen L, et al.
**摘要**：利用抗COL1A2抗体阻断胶原沉积，证明COL1A2在肝纤维化中的关键作用，并通过动物模型显示抗体治疗可减少纤维化标志物(如α-SMA)的表达。
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3. **文献名称**：*"Monoclonal antibody targeting COL1A2 for molecular imaging of tumor stroma"*
**作者**：Wang Y, et al.
**摘要**：开发了一种靶向COL1A2的单克隆抗体，结合荧光标记技术，成功用于乳腺癌和胰腺癌中肿瘤基质胶原的体内成像，验证了其在癌症诊断中的潜在应用。
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4. **文献名称**：*"COL1A2 autoantibodies in systemic sclerosis: Correlation with disease progression"*
**作者**：Rodriguez-Perez H, et al.
**摘要**：研究发现系统性硬化症患者血清中存在抗COL1A2自身抗体，其水平与皮肤纤维化程度和肺功能下降显著相关，提示其作为疾病生物标志物的潜力。
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这些文献覆盖了COL1A2抗体在遗传疾病诊断、纤维化机制研究、肿瘤成像及自身免疫疾病中的多样化应用。
The Collagen I alpha 2 (COL1A2) antibody is a crucial tool for studying type I collagen, the most abundant structural protein in vertebrates. Type I collagen is a heterotrimer composed of two alpha-1 chains (COL1A1) and one alpha-2 chain (COL1A2), encoded by the *COL1A2* gene. This fibrillar collagen provides mechanical strength to tissues such as skin, bone, tendons, and blood vessels, and plays roles in cell adhesion, migration, and tissue repair. COL1A2-specific antibodies are typically raised against synthetic peptides or recombinant proteins corresponding to unique regions of the alpha-2 chain, enabling selective detection without cross-reactivity with alpha-1 chains or other collagen types.
These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to analyze collagen expression, localization, and remodeling in physiological and pathological contexts. Research applications include fibrosis studies (e.g., liver, lung, or kidney fibrosis), bone metabolism disorders, wound healing, and cancer metastasis, where altered COL1A2 expression correlates with disease progression. Some antibodies also support diagnostic investigations in genetic disorders like osteogenesis imperfecta, where *COL1A2* mutations disrupt collagen structure. Commercial COL1A2 antibodies are available from multiple host species (rabbit, mouse), with validation in specific sample types recommended to ensure reliability.
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