| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADCADN; CXXC finger protein 9; CXXC9; DNA methyltransferase 1; DNA MTase; Dnmt1o; HSN1E; M.HsaI; MCMT; Met1; MommeD2 |
| Entrez GeneID | 1786 |
| WB Predicted band size | Calculated MW: 183 kDa; Observed MW: 183 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Dnmt1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Dnmt1抗体的3篇参考文献示例(内容为模拟概括，非真实文献)：
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1. **文献名称**：*DNA methyltransferase Dnmt1 associates with histone deacetylase activity*
**作者**：Robertson, K.D., et al.
**摘要**：该研究利用Dnmt1特异性抗体，通过免疫共沉淀技术揭示了Dnmt1与组蛋白去乙酰化酶(HDAC)的相互作用，表明DNA甲基化与染色质修饰之间存在功能关联，为表观遗传调控机制提供了证据。
2. **文献名称**：*Cell cycle-dependent localization of Dnmt1 in mammalian cells*
**作者**：Leonhardt, H., et al.
**摘要**：通过免疫荧光和Western blot分析，作者使用Dnmt1抗体证明其在细胞周期中的动态定位，发现Dnmt1在S期与复制焦点共定位，支持其在DNA复制过程中维持甲基化的作用。
3. **文献名称**：*Dnmt1 is essential for mouse development and methylation fidelity*
**作者**：Li, E., et al.
**摘要**：研究通过基因敲除小鼠模型结合Dnmt1抗体检测，发现Dnmt1缺失导致胚胎致死和基因组甲基化水平显著下降，强调了Dnmt1在发育和甲基化遗传中的必要性。
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(注：以上为示例性内容，实际文献需通过PubMed或学术数据库查询确认。)
Dnmt1 (DNA methyltransferase 1) is a critical enzyme responsible for maintaining DNA methylation patterns during DNA replication, ensuring the inheritance of epigenetic information across cell divisions. By catalyzing the transfer of methyl groups to cytosine residues in CpG dinucleotides, Dnmt1 preserves genomic stability, regulates gene expression, and participates in X-chromosome inactivation and imprinting. Dysregulation of Dnmt1 is linked to developmental abnormalities, cancer (via global hypomethylation or locus-specific hypermethylation), and neurological disorders.
Dnmt1 antibodies are essential tools for studying its expression, localization, and functional roles in epigenetic regulation. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Specific clones (e.g., 60B1220a, D63A9) are validated to recognize conserved epitopes across species, including human, mouse, and rat. Researchers rely on Dnmt1 antibodies to investigate its interplay with other epigenetic modifiers (e.g., HDACs, MeCP2) and its overexpression in malignancies like colorectal and hepatocellular carcinomas. Additionally, they help explore Dnmt1's role in cellular differentiation, aging, and responses to environmental stimuli. Quality validation (e.g., knockout controls, peptide blocking assays) ensures antibody specificity, minimizing cross-reactivity with paralogs like Dnmt3a/3b. Such reagents advance both basic research and therapeutic targeting of DNA methylation pathways.
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