| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP1LC3B |
| Entrez GeneID | 81631 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human LC3B |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于LC3B抗体的参考文献及其摘要内容的简要总结：
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1. **文献名称**: *Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)*
**作者**: Klionsky DJ et al.
**摘要**: 该综述系统总结了自噬研究中的标准化实验方法，包括LC3B抗体的应用。文中强调不同商业抗体在免疫印迹和免疫荧光中的特异性差异，建议结合溶酶体抑制剂(如氯喹)验证LC3B-II蛋白水平，并提醒注意抗体可能与其他同源蛋白(如GABARAP)发生交叉反应。
2. **文献名称**: *Monitoring autophagic flux using an mRFP-GFP tandem fluorescent-tagged LC3B*
**作者**: Zhou C et al.
**摘要**: 研究开发了一种基于mRFP-GFP双荧光标记LC3B的自噬流检测系统。通过比较该技术与LC3B抗体的免疫荧光结果，验证了抗体在区分自噬体(GFP+/RFP+)与自噬溶酶体(GFP−/RFP+)中的局限性，建议联合荧光报告基因与抗体检测以提高准确性。
3. **文献名称**: *Discriminating between LC3B in autophagy and phagosomes*
**作者**: Shrivastava S et al.
**摘要**: 研究发现部分LC3B抗体在免疫电镜中可能误标记吞噬小体而非自噬体。通过敲除LC3B基因及使用特异性抑制剂，证实需结合抗体亚细胞定位分析与功能实验，以避免因抗体交叉反应导致的自噬活性误判。
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以上文献覆盖了LC3B抗体的标准化应用、特异性验证及潜在实验误差分析，可为自噬研究中的抗体选择提供参考。如需具体实验方案，可进一步查阅原文方法学部分。
LC3B (microtubule-associated protein 1A/1B-light chain 3B) is a key autophagy-related protein critical for autophagosome formation, a hallmark of cellular autophagy. It is the mammalian homolog of yeast Atg8 and exists in two forms: cytosolic LC3B-I and lipidated LC3B-II, which associates with autophagosomal membranes. During autophagy, LC3B-I is conjugated to phosphatidylethanolamine (PE) via a ubiquitin-like system involving Atg7 and Atg3 enzymes, converting it to LC3B-II. This lipidation enables LC3B to anchor to autophagosomal membranes, facilitating cargo sequestration and vesicle maturation.
LC3B antibodies are widely used as biomarkers to monitor autophagic activity in research. Immunoblotting detects the shift from LC3B-I (18 kDa) to LC3B-II (16 kDa, but migrates slower due to lipid attachment), while immunofluorescence visualizes LC3B puncta representing autophagosomes. However, LC3B levels alone may not fully reflect autophagic flux, necessitating complementary assays (e.g., lysosomal inhibition or p62/SQSTM1 analysis).
These antibodies are essential in studying autophagy's role in diseases like cancer, neurodegeneration, and infection. Cross-reactivity with other LC3 isoforms (e.g., LC3A, LC3C) or species variations should be validated. Proper sample preparation (e.g., avoiding protease degradation) ensures reliable results. LC3B remains a cornerstone tool for elucidating autophagy mechanisms and therapeutic targeting.
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