| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD; SOD2 |
| Entrez GeneID | 6648 |
| WB Predicted band size | Calculated MW: 25 kDa; Observed MW: 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Synthetic peptide. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于SOD2抗体的3篇参考文献概览(文献信息为模拟示例，建议通过学术数据库核实)：
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1. **文献名称**：*Validation of a Specific SOD2 Antibody for Mitochondrial Superoxide Dismutase Detection*
**作者**：Smith J, et al.
**摘要**：该研究评估了一种新型SOD2抗体的特异性，通过Western blot和免疫荧光验证其在人类和小鼠组织中线粒体SOD2的检测效果，证明其优于其他商业抗体，并应用于氧化应激模型中的蛋白表达分析。
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2. **文献名称**：*SOD2 Immunohistochemical Staining in Cancer: Antibody Comparison and Clinical Implications*
**作者**：Lee H, et al.
**摘要**：研究比较了五种SOD2抗体在肿瘤组织中的染色效果，发现克隆号3A3的抗体在结直肠癌样本中特异性最强，可用于评估SOD2表达与患者预后的相关性。
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3. **文献名称**：*Mitochondrial Oxidative Stress and SOD2 Function: Insights from Knockout Mouse Models*
**作者**：Brown K, et al.
**摘要**：利用SOD2敲除小鼠模型，结合特异性抗体(ABCAM货号#12345)验证SOD2缺失对线粒体功能的影响，证实该抗体在组织切片和细胞裂解液中均具有高灵敏度和低交叉反应性。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词“SOD2 antibody validation”或“MnSOD antibody specificity”获取最新研究。
Superoxide dismutase 2 (SOD2), also known as manganese-dependent superoxide dismutase (MnSOD), is a critical mitochondrial enzyme that catalyzes the conversion of superoxide radicals into hydrogen peroxide and oxygen, thereby protecting cells from oxidative damage. As a primary antioxidant defense in mitochondria, SOD2 plays a vital role in maintaining redox balance and mitigating oxidative stress, which is implicated in aging, neurodegenerative diseases, cancer, and metabolic disorders.
SOD2 antibodies are essential tools for studying the expression, localization, and function of this enzyme in biological systems. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to quantify SOD2 levels in tissues or cells under varying physiological or pathological conditions. Researchers often validate SOD2 antibodies using knockout models or siRNA-mediated silencing to ensure specificity, as cross-reactivity with other SOD isoforms (e.g., cytosolic SOD1 or extracellular SOD3) must be excluded.
The development and application of SOD2 antibodies have advanced understanding of its regulatory mechanisms, including transcriptional control by NF-κB and FOXO3. and post-translational modifications like acetylation. Dysregulation of SOD2 is linked to diseases such as Alzheimer’s, Parkinson’s, and cancer, where its dual role as both a tumor suppressor and promoter highlights context-dependent functions. Reliable SOD2 antibodies thus remain crucial for exploring therapeutic strategies targeting oxidative stress pathways.
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