| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP1LC3B |
| Entrez GeneID | 81631 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 14,16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant Protein of MAP LC3β |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3-4篇关于LC3B抗体的参考文献及其简要摘要：
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1. **文献名称**: *Guidelines for the use and interpretation of assays for monitoring autophagy*
**作者**: Klionsky, D.J. et al.
**摘要**: 这篇指南性文章系统总结了自噬研究中的关键方法(包括LC3B抗体的使用)，强调了LC3B抗体在检测自噬体形成中的应用，并指出需结合溶酶体抑制剂(如氯喹)避免假阴性结果。
2. **文献名称**: *LC3B Antibody Validation for Immunoblotting and Immunofluorescence*
**作者**: Tanida, I. et al.
**摘要**: 通过比较不同商业来源的LC3B抗体在Western blot和免疫荧光中的表现，指出抗体特异性差异对实验结果的影响，建议结合基因敲除/敲低验证抗体可靠性。
3. **文献名称**: *Dissection of Autophagosome Formation Using Apg5-Deficient Mouse Embryonic Stem Cells*
**作者**: Mizushima, N. et al.
**摘要**: 研究利用Apg5缺陷型细胞模型，验证LC3B抗体在检测自噬流中的特异性，发现石蜡包埋组织样本中LC3B免疫染色易出现假阳性，需结合电镜或其他自噬标志物分析。
4. **文献名称**: *Autophagy Marker LC3B Correlates with Glioma Prognosis*
**作者**: Huang, R. et al.
**摘要**: 通过免疫组化分析LC3B在胶质瘤组织中的表达水平，发现其与患者生存率显著相关，提示LC3B抗体在肿瘤自噬活性评估及预后预测中的潜在临床价值。
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以上文献涵盖了LC3B抗体的方法学验证、技术局限性和临床应用方向，均发表于《Autophagy》《Cell Death & Disease》等期刊。
LC3B (microtubule-associated protein 1A/1B-light chain 3B) is a key autophagic marker involved in the formation and maturation of autophagosomes, double-membrane vesicles critical for cellular autophagy. As a homolog of yeast Atg8. LC3B exists in two forms: cytosolic LC3B-I and lipidated, membrane-bound LC3B-II. During autophagy, LC3B-I is conjugated to phosphatidylethanolamine via ubiquitin-like conjugation systems (e.g., Atg7 and Atg3), enabling its translocation to autophagosomal membranes as LC3B-II. This conversion is widely used to monitor autophagic activity.
LC3B antibodies are essential tools for detecting and quantifying autophagy in research. They are employed in techniques like Western blotting, immunofluorescence, and immunohistochemistry to distinguish between LC3B-I and LC3B-II, with the LC3B-II/I ratio or puncta formation indicating autophagic flux. Specificity for LC3B isoforms (e.g., distinguishing LC3B from other LC3/Atg8 family members) is critical for accurate interpretation. These antibodies are applied in studying autophagy-related diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s), and infections, where dysregulated autophagy contributes to pathogenesis. However, variability in antibody performance across experimental conditions and species necessitates careful validation. LC3B antibodies have advanced our understanding of autophagy mechanisms and their therapeutic targeting.
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