| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1 |
| Entrez GeneID | 5894 |
| WB Predicted band size | Calculated MW: 73 kDa; Observed MW: 73 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human C-RAF. AA range:251-300 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇涉及Raf1抗体的代表性文献摘要(虚构示例，仅供参考格式)：
1. **文献名称**: "Raf1 activation in cancer: Antibody-based detection and therapeutic implications"
**作者**: Smith J, et al.
**摘要**: 本研究利用特异性Raf1抗体(克隆号X)通过免疫印迹分析多种癌细胞系，发现Raf1在KRAS突变肿瘤中异常磷酸化，提示其作为治疗靶点的潜力。
2. **文献名称**: "Development of a phospho-specific Raf1 antibody for signaling pathway analysis"
**作者**: Lee H, et al.
**摘要**: 报道了一种新型抗Raf1 Ser338磷酸化抗体，验证了其在流式细胞术和免疫组化中的特异性，并用于检测EGFR信号通路中Raf1的激活动态。
3. **文献名称**: "Raf1 knockdown via antibody-mediated delivery of siRNA in vivo"
**作者**: Garcia R, et al.
**摘要**: 开发了靶向Raf1的抗体-siRNA偶联物，在小鼠模型中成功抑制肝细胞癌生长，证实抗体靶向递送策略的可行性。
(注：以上为模拟内容，实际文献需通过PubMed或Google Scholar以关键词“Raf1 antibody” + 研究领域检索。)
**Background of Raf1 Antibody**
The Raf1 antibody is a crucial tool in studying the Raf1 protein (also known as CRAF or c-Raf), a serine/threonine kinase central to the MAPK/ERK signaling pathway. This pathway regulates critical cellular processes, including proliferation, differentiation, and survival. Raf1 acts downstream of Ras proteins, forming part of a kinase cascade (Raf-MEK-ERK) that transmits extracellular signals to the nucleus. Dysregulation of Raf1. through overexpression or activating mutations, is implicated in cancer development, particularly in tumors with RAS or BRAF mutations.
Raf1 antibodies are widely used in research to detect Raf1 expression, localization, and activation status in cells and tissues. They enable techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study Raf1’s role in signaling dynamics, cancer progression, and drug resistance. Phospho-specific Raf1 antibodies (e.g., targeting pSer338/pSer339) are essential for assessing its activation during pathway stimulation.
These antibodies are also vital in therapeutic contexts, as Raf1 inhibitors are explored for cancers resistant to BRAF-targeted therapies. Validating Raf1 antibody specificity (via knockout controls) ensures accurate detection, avoiding cross-reactivity with related kinases like BRAF or ARAF. Commercial Raf1 antibodies are available from multiple vendors, often validated in diverse applications, supporting both basic research and preclinical studies targeting the MAPK pathway.
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