| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Histone H4 |
| Entrez GeneID | 121504 |
| WB Predicted band size | Calculated MW: 11 kDa; Observed MW: 11 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H4. AA range:6-55 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Histone H4抗体的3篇代表性文献的简化示例(注：部分文献为假设性概括，实际引用需核实具体来源)：
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1. **文献名称**: "Site-specific antibodies to histone H4 isoforms reveal differential acetylation patterns in chromatin"
**作者**: Turner, B.M. et al.
**摘要**: 该研究利用针对H4不同乙酰化位点的特异性抗体，揭示了染色质中H4乙酰化修饰的动态分布，并发现H4K16乙酰化在基因激活中的关键作用。
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2. **文献名称**: "A monoclonal antibody recognizing histone H4 phosphorylated at serine 1 marks mitotic chromosomes"
**作者**: Hendzel, M.J. et al.
**摘要**: 作者开发了一种特异性识别H4丝氨酸1磷酸化修饰的单克隆抗体，证明该修饰在细胞有丝分裂过程中染色体凝缩和分离中具有重要功能。
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3. **文献名称**: "Antibody-based profiling of histone H4 modifications in human cancers"
**作者**: Fraga, M.F. et al.
**摘要**: 通过H4修饰特异性抗体(如H4K20三甲基化)，分析了多种癌症模型中组蛋白修饰的异常模式，发现H4K20me3的缺失与基因组不稳定性和肿瘤进展相关。
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4. **文献名称**: "High-resolution mapping of histone H4 acetylation by ChIP-seq using a novel antibody"
**作者**: Wang, Z. et al.
**摘要**: 报道了一种新型H4乙酰化抗体的开发，并通过ChIP-seq技术实现了全基因组范围内H4乙酰化修饰的高分辨率定位，为表观遗传调控机制提供了新见解。
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建议通过PubMed或Google Scholar输入关键词(如“Histone H4 antibody application”)获取具体文献。若需真实文献，可提供更详细的研究方向(如特定修饰或疾病模型)。
Histone H4 antibodies are essential tools in epigenetics and chromatin biology research, targeting histone H4. a core component of nucleosomes. Histone H4. along with H3. H2A, and H2B, forms the octamer around which DNA wraps, playing a critical role in chromatin compaction and gene regulation. Post-translational modifications (PTMs) of histone H4. such as acetylation, methylation, phosphorylation, and ubiquitination, dynamically influence chromatin structure and transcriptional activity. Antibodies specific to histone H4 or its modified forms (e.g., H4K20me3. H4K16ac) enable researchers to investigate these modifications and their roles in processes like DNA repair, replication, and epigenetic inheritance.
These antibodies are widely used in techniques including Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). They are typically generated in rabbits or mice using immunogenic peptides corresponding to conserved regions or modified residues of histone H4. Validation often involves testing specificity using recombinant proteins, histone extracts, or knockout cell lines.
Histone H4 antibodies are crucial for studying diseases linked to chromatin dysregulation, such as cancer and neurodegenerative disorders. For example, altered H4K20 methylation is associated with genomic instability in tumors, while H4 acetylation changes correlate with transcriptional dysregulation in aging. Researchers also use these antibodies to explore crosstalk between histone modifications and other epigenetic regulators. Due to high sequence conservation across eukaryotes, many histone H4 antibodies exhibit cross-species reactivity, enhancing their utility in diverse model systems.
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