| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B |
| Entrez GeneID | 2932 |
| WB Predicted band size | Calculated MW: 47 kDa; Observed MW: 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GSK3B. AA range:1-50 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于GSK3β抗体的参考文献及其摘要概述：
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1. **文献名称**：*Glycogen synthase kinase-3 (GSK3): Regulation, actions, and diseases*
**作者**：Jope RS, Johnson GV
**摘要**：该综述总结了GSK3β在细胞信号通路(如Wnt和PI3K/AKT通路)中的核心作用，并讨论了其磷酸化调控机制。文中提到使用特异性GSK3β抗体(如针对Ser9磷酸化位点的抗体)检测其活性变化，及其在神经退行性疾病和糖尿病研究中的应用。
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2. **文献名称**：*GSK3β in immune response and inflammation: Mechanisms and therapeutic potential*
**作者**：Beurel E, Grieco SF, Jope RS
**摘要**：本文探讨了GSK3β在炎症和自身免疫疾病中的作用，重点通过Western blot和免疫荧光技术，使用磷酸化特异性抗体(如Tyr216抗体)分析免疫细胞中GSK3β的激活状态，揭示其作为治疗靶点的潜力。
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3. **文献名称**：*Subcellular distribution of GSK-3β in neuronal cells: A study using isoform-specific antibodies*
**作者**：Hughes K, Nikolakaki E, Plyte SE et al.
**摘要**：该研究开发了针对GSK3β亚型的特异性抗体，用于分析其在不同脑区神经元中的亚细胞定位。通过免疫组化和共聚焦显微镜，发现GSK3β在胞浆和突触中的富集，提示其在神经可塑性中的功能。
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这些文献涵盖了GSK3β抗体的应用场景(如活性检测、亚型区分和疾病机制研究)，适用于信号通路、神经科学及免疫学领域的研究参考。
Glycogen synthase kinase-3 beta (GSK3β) is a serine/threonine kinase involved in critical cellular processes, including metabolism, proliferation, differentiation, and apoptosis. It regulates key signaling pathways, such as Wnt/β-catenin, PI3K/Akt, and NF-κB, by phosphorylating substrates like β-catenin, tau, and cyclin D1. Dysregulation of GSK3β activity is linked to diseases like Alzheimer’s, diabetes, cancer, and mood disorders.
GSK3β antibodies are essential tools for studying its expression, localization, and activity in research. These antibodies detect total GSK3β or its post-translational modifications, particularly phosphorylation at Ser9 (inactivating) or Tyr216 (activating), which reflect its functional state. Monoclonal antibodies offer high specificity, while polyclonal antibodies may detect broader epitopes. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP) to investigate GSK3β’s role in disease models or signaling cascades. Validation via knockout controls or siRNA-mediated silencing ensures antibody specificity.
Given GSK3β’s dual role as a tumor suppressor or promoter depending on context, its antibodies are pivotal in cancer research. Additionally, they aid in studying neurodegenerative diseases, where hyperactive GSK3β contributes to tau hyperphosphorylation. These antibodies thus support both basic research and therapeutic development targeting GSK3β pathways.
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