| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CCNA1; Cyclin-A1 |
| Entrez GeneID | 8900 |
| WB Predicted band size | Calculated MW: 52 kDa; Observed MW: 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cyclin A. AA range:221-270 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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1. **"Cyclin A1 is a leukemia-associated antigen in acute myeloid leukemia"** by Yang, R. et al.
摘要：研究揭示了Cyclin A1在急性髓系白血病细胞中的异常高表达，并利用特异性抗体证实其作为潜在白血病标志物的作用，探讨了其在细胞周期失调中的机制。
2. **"Aberrant expression of cyclin A1 in human prostate cancer"** by Sweeney, C. et al.
摘要：通过Cyclin A1抗体检测发现，该蛋白在前列腺癌组织中显著过表达，且受雄激素信号调控，可能通过促进G2/M期转换驱动肿瘤进展。
3. **"Targeted disruption of the cyclin A1 gene leads to impaired germ cell development"** by Liu, D. et al.
摘要：利用基因敲除小鼠模型结合Cyclin A1抗体验证，发现该蛋白对精子形成至关重要，缺失导致减数分裂阻滞和雄性不育。
4. **"Cyclin A1 regulates HIV-1 replication through phosphorylation of viral proteins"** by Hofmann, H. et al.
摘要：研究通过抗体介导的蛋白互作实验，揭示Cyclin A1/CDK2复合物通过磷酸化HIV衣壳蛋白促进病毒复制，为抗病毒靶点提供新思路。
Cyclin A1 is a key regulatory protein belonging to the cyclin family, which plays a critical role in cell cycle progression, particularly during the S and G2/M phases. Unlike its more widely expressed homolog Cyclin A2. Cyclin A1 is predominantly expressed in germ cells and certain somatic tissues, with elevated levels observed in various cancers, including leukemia and solid tumors. It interacts with cyclin-dependent kinases (CDKs), such as CDK2 and CDK1. to regulate DNA replication, transcriptional modulation, and checkpoint control. Dysregulation of Cyclin A1 has been linked to genomic instability and oncogenesis, making it a potential therapeutic target.
Antibodies targeting Cyclin A1 are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to investigate cell cycle dynamics, cancer biology, and reproductive health. Specificity validation (e.g., via knockout controls or siRNA knockdown) is crucial, as cross-reactivity with Cyclin A2 or other family members can occur. Researchers also employ Cyclin A1 antibodies to explore its role in drug resistance, stem cell regulation, and DNA damage response pathways. Proper experimental optimization, including tissue fixation and antibody dilution, is necessary to ensure reliable results in diverse sample types.
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