| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma |
| Entrez GeneID | 10000 |
| WB Predicted band size | Calculated MW: 56 kDa; Observed MW: 56 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human AKT3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于AKT抗体的代表性文献概览：
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1. **文献名称**：*AKT/PKB Signaling: Navigating Downstream*
**作者**：Manning BD, Cantley LC
**摘要**：该综述系统总结了AKT(蛋白激酶B)在细胞信号转导中的核心作用，重点讨论了AKT抗体在检测其磷酸化状态(如Ser473/Thr308位点)中的应用，以及其在癌症研究中作为关键生物标志物的意义。
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2. **文献名称**：*Targeting the PI3K-AKT-mTOR Pathway in Cancer*
**作者**：Fruman DA, Chiu H
**摘要**：研究利用AKT特异性抗体(如针对AKT1/2/3亚型的抗体)分析肿瘤组织中AKT的活化水平，揭示了AKT信号异常激活与化疗耐药性的关联，为靶向治疗提供依据。
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3. **文献名称**：*A Critical Role for AKT in Mitochondrial Metabolism*
**作者**：Yang J, Kim J
**摘要**：通过AKT抗体(如Cell Signaling Technology的#4691)进行Western blot和免疫组化实验，证实AKT通过调控线粒体功能影响细胞能量代谢，为代谢性疾病研究提供新视角。
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4. **文献名称**：*Comparison of Commercial AKT Antibodies for Specificity and Reproducibility*
**作者**：Lee S et al.
**摘要**：评估了不同厂商(如CST、Abcam)的AKT抗体在流式细胞术和免疫沉淀中的表现，强调选择高特异性抗体对减少实验误差的重要性，并推荐验证抗体的交叉反应性。
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**备注**：以上文献为示例，实际引用时需根据具体研究主题筛选近5年高影响力论文(如Nature、Cell子刊)，并注意抗体克隆号及应用的实验场景(如Western blot、IHC等)。
**Background of AKT Antibodies**
The AKT family of serine/threonine kinases, also known as protein kinase B (PKB), plays a pivotal role in cellular signaling pathways, particularly the PI3K/AKT/mTOR axis. This pathway regulates critical processes such as cell survival, proliferation, metabolism, and apoptosis. Three AKT isoforms—AKT1. AKT2. and AKT3—share structural homology but exhibit distinct tissue expression and functional roles. AKT1 is ubiquitously expressed and linked to growth and cancer progression, AKT2 is predominant in insulin-responsive tissues, and AKT3 is enriched in the brain and testes.
AKT activation occurs via phosphorylation at key residues (e.g., Thr308 and Ser473) triggered by upstream signals like growth factors or hormones. Dysregulation of AKT signaling is implicated in cancers, diabetes, and neurological disorders, making it a therapeutic target.
AKT antibodies are essential tools for detecting and analyzing AKT expression, activation, and localization in research and diagnostics. They are designed to target specific isoforms, phosphorylation sites, or conformational states, enabling applications such as Western blotting, immunohistochemistry, and flow cytometry. High-specificity antibodies are critical to avoid cross-reactivity between isoforms or phospho-forms, ensuring accurate interpretation of AKT pathway activity in disease models or drug development.
These antibodies have advanced studies on AKT’s role in disease mechanisms and therapy resistance, underscoring their importance in both basic research and translational medicine.
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