| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | OMGP |
| WB Predicted band size | 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human OMG |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于LEF1抗体的3篇文献示例(注：内容为模拟概括，非真实文献)：
1. **"LEF1 Antibody Validation in Wnt Signaling Pathway Studies"**
- **作者**: Smith A, et al.
- **摘要**: 本文验证了一种特异性LEF1抗体在Western blot和免疫组化中的应用，确认其在检测Wnt信号通路活性中的可靠性，并用于结直肠癌组织样本分析。
2. **"Role of LEF1 in Pulmonary Fibrosis: Insights from Immunohistochemical Analysis"**
- **作者**: Chen B, et al.
- **摘要**: 通过使用LEF1抗体进行免疫组化实验，研究发现LEF1在肺纤维化患者的成纤维细胞中高表达，提示其可能作为治疗靶点。
3. **"Development of a Monoclonal LEF1 Antibody for Cancer Research"**
- **作者**: Lee C, et al.
- **摘要**: 研究团队开发了一种高特异性小鼠单克隆LEF1抗体，验证了其在流式细胞术和免疫荧光中的应用，并证明其在急性髓系白血病细胞核定位中的检测效果。
如需具体文献，建议通过PubMed或Google Scholar搜索关键词“LEF1 antibody”或“LEF1 immunohistochemistry”获取最新研究。
The LEF1 (Lymphoid Enhancer-Binding Factor 1) antibody is a crucial tool for studying the role of LEF1. a member of the T-cell factor/lymphoid enhancer factor (TCF/LEF) family of transcription factors. LEF1 is a key mediator of the Wnt/β-catenin signaling pathway, which regulates gene expression involved in cell proliferation, differentiation, and embryonic development. Structurally, LEF1 contains a β-catenin-binding domain and a high-mobility group (HMG) box that facilitates DNA binding, enabling it to activate target genes such as c-MYC and cyclin D1 when Wnt signaling is activated.
LEF1 plays pivotal roles in immune system development, particularly in T-cell maturation, and in tissue morphogenesis. Dysregulation of LEF1 is implicated in cancers, including colorectal carcinoma, leukemia, and breast cancer, where aberrant Wnt signaling drives tumor progression. The LEF1 antibody is widely used in research to detect LEF1 expression and localization via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). It helps identify nuclear LEF1 accumulation, a hallmark of activated Wnt signaling in cancer.
Researchers also utilize LEF1 antibodies to explore its interaction with β-catenin and other co-regulators, providing insights into transcriptional mechanisms. Commercial antibodies are typically validated for specificity across human, mouse, and rat samples. Studies linking LEF1 overexpression to poor prognosis in certain cancers underscore its therapeutic relevance, making this antibody vital for both basic research and clinical investigations.
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