| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 5' ' -AMP-activated protein kinase subunit beta-1; AMP-activated; noncatalytic; beta-1; AMPK; AMPK beta 1 chain; AMPK subunit beta-1; AMPK-BETA-1; AMPKb; HAMPKb; PRKAB1 |
| Entrez GeneID | 5564 |
| WB Predicted band size | Calculated MW: 30 kDa; Observed MW: 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human AMPK beta 1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与AMPK β1抗体相关的文献摘要简述：
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1. **文献名称**: "AMPK β1 deficiency reduces mouse lifespan in a sex-dependent manner"
**作者**: Salt, I.P. et al.
**摘要**: 本研究利用AMPK β1基因敲除小鼠模型，通过特异性AMPK β1抗体验证组织亚基表达缺失，发现β1缺失导致代谢紊乱并缩短雄性小鼠寿命，提示抗体在蛋白功能研究中的有效性。
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2. **文献名称**: "Structural basis for AMPK activation by small molecule agonists"
**作者**: Xiao, B. et al.
**摘要**: 通过X射线晶体学解析AMPK β1亚基结构，使用商业AMPK β1抗体进行免疫印迹验证重组蛋白表达，为药物靶向β1亚基的机制研究提供工具支持。
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3. **文献名称**: "Tissue-specific distribution of AMPK β1 and β2 isoforms in metabolic regulation"
**作者**: Steinberg, G.R. et al.
**摘要**: 采用亚型特异性AMPK β1抗体分析小鼠心脏、肝脏等组织，证实β1在心肌中高表达，并揭示其与脂代谢通路的关联，突显抗体在亚细胞定位研究中的重要性。
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(注：上述文献信息为模拟生成，实际文献需通过PubMed等数据库检索确认。)
**Background of AMPK Beta 1 Antibody**
AMP-activated protein kinase (AMPK) is a critical energy sensor regulating cellular metabolism to maintain energy homeostasis. It exists as a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. The β subunit (β1 or β2) acts as a scaffold, linking α and γ subunits, and plays a role in substrate recognition and cellular localization. AMPK beta 1 (PRKAB1), encoded by the *PRKAB1* gene, is ubiquitously expressed and essential for AMPK’s metabolic functions, including glucose uptake, lipid metabolism, and mitochondrial biogenesis.
AMPK beta 1 antibodies are tools designed to detect and quantify the β1 subunit in various experimental settings, such as Western blotting, immunohistochemistry, or immunofluorescence. These antibodies aid in studying AMPK’s activation status, tissue-specific expression, and interactions under conditions like metabolic stress, exercise, or disease states (e.g., diabetes, obesity, cancer). Beta 1-specific antibodies are particularly valuable for distinguishing between β1 and β2 isoforms, which may have divergent roles in different tissues or pathologies.
Research using AMPK beta 1 antibodies has advanced understanding of metabolic disorders, aging, and therapeutic interventions (e.g., metformin). Their specificity and reliability are crucial for elucidating AMPK’s complex regulatory mechanisms and its potential as a drug target.
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