| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MCC1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human MCC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ATG5/APG5L抗体的3篇代表性文献摘要：
1. **"Development of a novel monoclonal antibody specific for ATG5"**
*Authors: Mizushima N, Yoshimori T, Levine B*
摘要：该研究报道了一种针对哺乳动物ATG5蛋白的高特异性单克隆抗体的开发，验证了其在免疫印迹和免疫荧光中对自噬体的检测效果，并应用于自噬通量分析。
2. **"Autophagy monitors antigen trafficking through ATG5-dependent vesicular formation"**
*Authors: Schmid D, Pypaert M, Münz C*
摘要：研究利用ATG5抗体在免疫电镜中定位自噬相关结构，揭示了ATG5在抗原呈递囊泡形成中的作用，抗体特异性通过ATG5敲除细胞系验证。
3. **"Impaired autophagy in human plaque-derived macrophages by oxidized lipids involves ATG5 dysfunction"**
*Authors: Razani B, Feng C, Coleman-Derr D*
摘要：通过ATG5抗体检测动脉粥样硬化患者巨噬细胞中自噬水平，发现氧化脂质通过抑制ATG5-ATG12复合物形成干扰自噬，抗体用于Western blot和流式细胞术定量分析。
注：APG5L为酵母自噬基因APG5的哺乳动物同源基因，标准名称为ATG5.上述文献均使用ATG5抗体研究自噬机制及疾病关联。
The APG5L antibody targets the autophagy-related protein 5 (ATG5), a critical component of the autophagy pathway, a conserved cellular process for degrading and recycling cytoplasmic components. ATG5 plays a pivotal role in autophagosome formation by conjugating with ATG12. forming a complex essential for the lipidation of microtubule-associated protein 1A/1B-light chain 3 (LC3). This lipidation step is crucial for membrane elongation and autophagosome maturation, enabling the sequestration of cellular cargo for lysosomal degradation.
APG5L antibodies are widely used in research to detect ATG5 expression and monitor autophagy dynamics in various experimental models, including immunoblotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC). These antibodies help elucidate autophagy's role in physiological processes (e.g., development, nutrient sensing) and pathological conditions such as cancer, neurodegenerative diseases, and infections. Dysregulation of ATG5-mediated autophagy is linked to tumor progression, neurodegeneration (e.g., Alzheimer’s, Parkinson’s), and immune responses, making it a therapeutic target.
Researchers also employ APG5L antibodies to validate autophagy modulation by drugs, genetic interventions, or environmental stressors. Their specificity and reliability are critical for distinguishing baseline autophagy from stress-induced activation. As autophagy gains prominence in disease mechanisms and treatment strategies, APG5L antibodies remain indispensable tools for advancing molecular and cellular studies in both basic and translational research.
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