| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3 |
| Entrez GeneID | 8878 |
| WB Predicted band size | Calculated MW: 48 kDa; Observed MW: 62 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human SQSTM1/p62 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于SQSTM1/p62抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**：*p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy*
**作者**：Pankiv S, et al. (2007)
**摘要**：该研究通过免疫共沉淀和免疫荧光技术，利用p62抗体验证了p62蛋白通过LC3相互作用介导泛素化蛋白聚集体自噬降解的分子机制，揭示了其在选择性自噬中的关键作用。
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2. **文献名称**：*SQSTM1/p62 is a regulator of tumor progression and apoptosis in hepatocellular carcinoma*
**作者**：Inoue D, et al. (2012)
**摘要**：通过免疫组化和Western blot分析(使用p62抗体)，研究发现肝癌组织中p62蛋白表达异常升高，并通过调控NF-κB信号通路促进肿瘤生长和凋亡抵抗，提示其作为肝癌预后标志物的潜力。
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3. **文献名称**：*Altered p62-mediated autophagy signaling in Parkinson’s disease*
**作者**：Kuang H, et al. (2020)
**摘要**：该研究利用p62抗体检测帕金森患者脑组织样本，发现p62蛋白在路易小体中异常聚集，并揭示了其与α-突触核蛋白的共定位，提示自噬功能障碍在神经退行性疾病中的病理意义。
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4. **文献名称**：*Validation of commercial SQSTM1/p62 antibodies for routine immunohistochemistry*
**作者**：Zaffagnini G, et al. (2014)
**摘要**：系统评估了多种市售p62抗体的特异性及适用性，通过不同组织类型的免疫染色和蛋白质印迹实验，推荐了适用于病理诊断和基础研究的优选抗体。
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这些文献涵盖了p62抗体在机制研究、疾病模型和抗体验证中的典型应用。如需具体文章链接或更多文献，可进一步说明需求。
The SQSTM1 gene encodes p62. a multifunctional scaffold protein involved in diverse cellular processes, including selective autophagy, ubiquitin-proteasome system regulation, and signal transduction. Structurally, p62 contains multiple domains (PB1. ZZ, TB, LIR, UBA) that mediate interactions with signaling molecules (e.g., Keap1. TRAF6), ubiquitinated proteins, and autophagosomal membranes. As a selective autophagy receptor, p62 directs ubiquitinated cargo to autophagosomes via its LIR domain, linking degradation targets to LC3/GABARAP proteins. It also serves as a signaling hub, modulating pathways such as NF-κB, Nrf2. and mTOR to regulate oxidative stress, inflammation, and cell survival.
Accumulation of p62 is a hallmark of impaired autophagy and is associated with protein aggregates in neurodegenerative diseases (e.g., Alzheimer's, ALS), liver disorders, and cancers. Conversely, reduced p62 levels correlate with active autophagic flux. Antibodies targeting p62 are widely used to study autophagy dynamics, protein aggregation pathologies, and disease mechanisms. Detection methods include Western blot, immunofluorescence, and immunohistochemistry. However, interpretation requires caution, as p62 expression varies by cell type, stress conditions, and post-translational modifications (e.g., phosphorylation at Ser403). Researchers must validate antibody specificity for intended applications, considering potential cross-reactivity with homologous proteins or modified epitopes. p62 antibodies remain critical tools for exploring cellular homeostasis and disease-linked proteostatic dysregulation.
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