WB | 咨询技术 | Human,Mouse,Rat |
IF | 1/20 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 1/50-1/200 | Human,Mouse,Rat |
FCM | 1/50-1/100 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | L40; CEP52; RPL40; HUBCEP52 |
Entrez GeneID | 7311 |
WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 10 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Rat |
Immunogen | A synthetic peptide of human UBA52 |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Ubiquitin 60S Ribosomal Protein L40(RPL40)抗体的3篇参考文献,按文献名称、作者和摘要内容简要概括:
1. **文献名称**:*"Ubiquitin fusion proteins are overexpressed in colon cancer and induce p53 stabilization"*
**作者**:Smith J, et al.
**摘要**:该研究利用抗RPL40抗体分析了结肠癌组织中泛素融合蛋白的表达水平,发现其过表达与p53蛋白稳定性增强相关,揭示了RPL40在肿瘤发生中的潜在调控机制。
2. **文献名称**:*"Ribosomal protein L40 is essential for translational elongation and stress response in yeast"*
**作者**:Chen L, Wang H.
**摘要**:通过抗RPL40抗体的免疫沉淀实验,作者发现酵母中RPL40缺失导致核糖体功能异常,影响翻译延伸过程,并降低细胞在应激条件下的存活率。
3. **文献名称**:*"The ubiquitin-60S ribosomal protein L40 axis regulates autophagy flux"*
**作者**:Kim S, et al.
**摘要**:该研究使用RPL40抗体进行免疫荧光染色,证明泛素化修饰的RPL40通过调控自噬关键蛋白LC3的加工,影响细胞自噬活性,为癌症治疗提供新靶点。
注:以上文献信息为示例,实际引用时建议通过PubMed或学术数据库检索具体文献(如输入关键词:RPL40 antibody、Ubiquitin ribosomal protein L40)。若需近期研究,可关注2020年后发表的论文。
The ubiquitin 60S ribosomal protein L40 (Ub-L40) antibody targets a unique hybrid protein formed by the fusion of ubiquitin, a highly conserved regulatory protein, and ribosomal protein L40. a component of the 60S ribosomal subunit. Ubiquitin is typically involved in protein degradation via the ubiquitin-proteasome system, while L40 contributes to ribosome assembly and translational machinery. In humans, the *UBB* and *UBC* genes encode ubiquitin precursors that are post-translationally processed to release free ubiquitin. However, certain ribosomal proteins, including L40 (encoded by *RPL40*), are transcribed as ubiquitin fusion genes (e.g., *UBA52*), where ubiquitin and L40 are co-expressed from the same mRNA before proteolytic cleavage.
Ub-L40 antibodies are critical tools for studying the dual roles of ubiquitin-ribosomal fusion proteins in cellular processes such as protein turnover, ribosome biogenesis, and stress responses. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect Ub-L40 expression, localization, and interactions. Dysregulation of Ub-L40 has been implicated in diseases like cancer and neurodegeneration, where disrupted proteostasis or ribosomal function contributes to pathology. These antibodies also aid in exploring crosstalk between protein degradation pathways and translational regulation, offering insights into cellular adaptation mechanisms.
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