| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARH12; ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC |
| Entrez GeneID | 387/388/389 |
| WB Predicted band size | Calculated MW: 22 kDa; Observed MW: 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human Rho C |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于RhoA/B/C抗体的参考文献示例(注：以下为模拟内容，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *"Selective antibody-based inhibition of RhoA, RhoB, and RhoC reveals distinct roles in cell migration and invasion"*
**作者**: Smith J, Doe R, Garcia-Mata R
**摘要**: 本文通过开发针对RhoA、RhoB和RhoC的特异性单克隆抗体，验证了其在Western blot和免疫荧光中的亚型特异性，并证明不同Rho亚型在癌细胞迁移和侵袭中的独特功能。
2. **文献名称**: *"Characterization of commercially available Rho GTPase antibodies for specificity and cross-reactivity"*
**作者**: Lee H, Johnson A, Hall A
**摘要**: 系统评估了市售RhoA/B/C抗体的特异性，发现部分抗体在RhoB和RhoC间存在交叉反应，强调实验前需通过基因敲除或siRNA验证抗体可靠性。
3. **文献名称**: *"RhoC-specific monoclonal antibody development and its application in metastatic cancer studies"*
**作者**: Chen X, Wang Y, Weinberg RA
**摘要**: 报道了一种高特异性抗RhoC单克隆抗体的开发，应用于转移性肿瘤组织样本的免疫组化分析，揭示RhoC表达与患者预后的相关性。
4. **文献名称**: *"Differential roles of RhoA and RhoC in vascular smooth muscle contraction: Insights from antibody-mediated functional blocking"*
**作者**: Brown KA, Somlyo AV
**摘要**: 利用特异性抗体阻断RhoA和RhoC的功能，发现两者在血管平滑肌收缩中的不同调控机制，提示亚型特异性抗体在功能研究中的关键作用。
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**备注**：实际文献需通过PubMed、Google Scholar等平台检索关键词(如“RhoA antibody specificity”“RhoC antibody validation”)获取。部分经典研究可能由Rho GTPase领域专家(如Alan Hall、Klaus Aktories)发表。建议结合实验需求(如应用场景、物种来源)筛选文献。
RhoA, RhoB, and RhoC are closely related small GTPases within the Rho subfamily of Ras superfamily proteins, playing critical roles in regulating cytoskeletal dynamics, cell migration, proliferation, and gene expression. Despite sharing ~85% amino acid sequence identity, they exhibit distinct cellular functions and localization. RhoA is ubiquitously expressed and involved in stress fiber formation; RhoB localizes to endosomes and modulates vesicular trafficking and apoptosis; RhoC is associated with cancer invasion and metastasis.
Antibodies targeting RhoA/B/C are essential tools for studying their expression, activation, and subcellular distribution. Due to high homology (especially between RhoA and RhoC, which differ by only 13 residues), generating isoform-specific antibodies is challenging. Most commercial antibodies are raised against unique peptide sequences in variable regions (e.g., RhoA's C-terminal hypervariable region) to minimize cross-reactivity. Validation often includes knockout cell lines or peptide blocking assays to confirm specificity.
These antibodies are widely used in techniques like Western blot, immunofluorescence, and immunohistochemistry to investigate Rho protein dysregulation in cancer, neurological disorders, and cardiovascular diseases. Researchers must carefully select antibodies validated for their specific applications, as nonspecific binding can lead to misinterpretation, particularly given overlapping roles of Rho isoforms in signaling pathways.
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