| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAP; CAP1-PEN |
| Entrez GeneID | 10487 |
| WB Predicted band size | Calculated MW: 52 kDa; Observed MW: 56 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human CAP1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于CAP1抗体的3篇模拟参考文献示例(注：内容为假设性概括，实际文献需通过学术数据库验证)：
---
1. **标题**: *"CAP1 Antibody Reveals Its Role in Actin Dynamics and Cancer Cell Migration"*
**作者**: Smith J, et al.
**期刊**: *Cell Biology International*, 2020
**摘要**: 本研究利用特异性CAP1抗体，证实了CAP1蛋白通过调控肌动蛋白细胞骨架重组促进乳腺癌细胞迁移，为靶向CAP1的癌症治疗策略提供依据。
2. **标题**: *"Development of a Monoclonal CAP1 Antibody for Neurodegenerative Disease Research"*
**作者**: Li X, et al.
**期刊**: *Journal of Neuroscience Methods*, 2019
**摘要**: 文章报道了一种新型CAP1单克隆抗体的开发，并应用于阿尔茨海默病模型小鼠脑组织分析，发现CAP1与β-淀粉样蛋白沉积存在共定位现象。
3. **标题**: *"CAP1 Antibody-Based Detection of Protein-Protein Interactions in cAMP Signaling Pathways"*
**作者**: Müller R, et al.
**期刊**: *Biochemical Journal*, 2021
**摘要**: 通过免疫共沉淀(Co-IP)结合CAP1抗体，揭示了CAP1与腺苷酸环化酶的相互作用机制，阐明了其在cAMP-PKA信号通路中的调控作用。
---
**注意**：以上为模拟示例，实际文献需通过PubMed、Web of Science等平台检索确认。建议以“CAP1 antibody”、“Cyclase-associated protein 1”等关键词结合研究领域(如癌症、神经科学)进行精准查询。
CAP1 (adenylyl cyclase-associated protein 1), also known as CAP/PESP1. is a conserved cytoskeletal regulatory protein involved in actin dynamics, signal transduction, and cellular processes like migration, endocytosis, and vesicle trafficking. Initially identified in yeast for its interaction with adenylyl cyclase, mammalian CAP1 regulates actin filament turnover by promoting cofilin-mediated actin depolymerization. It contains an N-terminal helical domain for adenylyl cyclase binding and a C-terminal region for actin/cofilin interaction. CAP1 is ubiquitously expressed and plays roles in immune responses, glucose metabolism, and cancer progression.
CAP1 antibodies are essential tools for studying its localization, expression, and function. Research shows CAP1 overexpression in tumors (e.g., breast, pancreatic, and colorectal cancers) correlates with metastasis and poor prognosis, likely due to enhanced cell motility. In metabolic disorders, CAP1 interacts with insulin receptors, influencing glucose uptake. Antibodies targeting CAP1 (e.g., monoclonal clones like EPR8592 or polyclonal variants) are used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Recent studies also explore CAP1 as a therapeutic target, with antibodies aiding in mechanistic insights or diagnostic applications. However, functional redundancy with CAP2 and tissue-specific roles necessitate careful experimental validation of antibody specificity.
×
