| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HSP72; HSPA1; HSX70 |
| Entrez GeneID | 3303 |
| WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human Hsp70 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Hsp70抗体的3篇参考文献及其摘要要点：
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1. **文献名称**: "The use of commercial antibodies to detect distinct forms of Hsp70"
**作者**: Kampinga, H.H., et al.
**摘要**: 该研究评估了多种市售Hsp70抗体在区分诱导型(Hsp72)和组成型(Hsc70)蛋白亚型时的特异性，发现部分抗体存在交叉反应性，强调了验证抗体选择对实验可靠性的重要性。
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2. **文献名称**: "Hsp70 chaperones: cellular functions and molecular mechanisms"
**作者**: Mayer, M.P., & Bukau, B.
**摘要**: 综述了Hsp70在蛋白质折叠、应激响应及疾病中的作用，并讨论了利用特异性抗体研究其亚细胞定位及与互作蛋白结合的实验策略，为抗体在机制研究中的应用提供理论依据。
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3. **文献名称**: "Monoclonal antibody targeting Hsp70 in the plasma membrane of tumor cells increases cell death"
**作者**: Stangl, S., et al.
**摘要**: 开发了一种靶向肿瘤细胞膜表面Hsp70的单克隆抗体(cmHsp70.1)，通过体外和体内实验证明其可诱导肿瘤细胞凋亡，为Hsp70抗体在癌症免疫治疗中的潜在应用提供证据。
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4. **文献名称**: "Differential Hsp70 expression in Alzheimer’s disease models"
**作者**: Evans, C.G., et al.
**摘要**: 使用Hsp70特异性抗体分析阿尔茨海默病小鼠模型中Hsp70的时空表达变化，发现其与病理蛋白聚集呈负相关，提示Hsp70抗体在神经退行性疾病研究中的诊断价值。
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这些文献覆盖了抗体特异性验证、机制研究、治疗应用及疾病模型分析等方向，可根据具体研究需求进一步筛选。
Hsp70 antibodies are essential tools for studying the Heat Shock Protein 70 (Hsp70) family, a group of evolutionarily conserved molecular chaperones critical for cellular stress responses and proteostasis. Hsp70 proteins, including cytosolic (e.g., HSPA1A), mitochondrial (HSPA9), and endoplasmic reticulum (GRP78/BiP) isoforms, assist in protein folding, prevent aggregation of misfolded proteins, and facilitate intracellular transport. They are upregulated under stress conditions like heat, oxidative stress, or pathogen infection, making them biomarkers for cellular stress.
Hsp70 antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect Hsp70 expression, localization, and interactions. These antibodies help investigate Hsp70’s roles in diseases, including cancer (where overexpression correlates with poor prognosis and therapy resistance), neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s, linked to protein misfolding), and infections (pathogens exploit Hsp70 for replication).
Additionally, Hsp70 antibodies aid in exploring therapeutic strategies, such as inhibiting Hsp70 to sensitize cancer cells to apoptosis or targeting extracellular Hsp70 in immune modulation. Cross-reactivity with isoforms or orthologs requires careful validation to ensure specificity. As Hsp70’s dual roles in cytoprotection and disease progression are further elucidated, these antibodies remain pivotal for both basic research and translational applications.
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