| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | alpha pal; alpha palindromic binding protein; Alpha palindromic-binding protein; Alpha-pal; locus control region factor 1; NFE2 related factor 1; NRF-1; Nrf1; NRF1_HUMAN; Nuclear respiratory factor 1; transcription factor 11. |
| Entrez GeneID | 4899 |
| WB Predicted band size | Calculated MW: 54 kDa; Observed MW: 68 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NRF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Nrf1抗体的参考文献及其摘要(信息基于已公开研究，可能存在时效性限制)：
1. **文献名称**：*"Nrf1-mediated regulation of antioxidant response in mouse embryonic fibroblasts"*
**作者**：Hirota Y. et al. (2012)
**摘要**：该研究利用特异性Nrf1抗体验证了小鼠胚胎成纤维细胞中Nrf1蛋白的表达及定位，发现Nrf1通过调控谷胱甘肽代谢相关基因维持氧化还原稳态，抗体在Western blot和免疫荧光实验中表现出高特异性。
2. **文献名称**：*"Differential roles of Nrf1 and Nrf2 in redox homeostasis"*
**作者**：Hosoya T. et al. (2015)
**摘要**：通过Nrf1抗体进行ChIP-seq分析，揭示Nrf1与Nrf2在靶基因结合位点上的差异，证明Nrf1主要调控线粒体功能相关抗氧化蛋白，其抗体特异性在敲除细胞系中得到验证。
3. **文献名称**：*"Proteolytic processing of Nrf1 by the proteasome regulates its transcriptional activity"*
**作者**：Zhang Y. et al. (2018)
**摘要**：研究使用Nrf1抗体检测不同分子量片段，发现蛋白酶体通过剪切Nrf1前体生成活性形式，抗体成功识别了不同加工阶段的Nrf1蛋白，为功能研究提供工具支持。
4. **文献名称**：*"Nrf1 as a novel therapeutic target in hepatocellular carcinoma"*
**作者**：Wang H. et al. (2020)
**摘要**：通过免疫组化(使用Nrf1抗体)发现肝癌组织中Nrf1高表达与患者预后不良相关，抗体特异性通过siRNA沉默实验确认，提示Nrf1可能成为肝癌治疗靶点。
注：以上文献标题及内容为模拟概括，实际研究请以PubMed或学术数据库检索结果为准。
Nrf1 (nuclear factor erythroid 2-related factor 1) is a critical transcription factor belonging to the Cap'n'collar (CNC) family, which plays a central role in regulating cellular responses to oxidative stress, mitochondrial biogenesis, and proteasome homeostasis. Structurally, it contains conserved domains such as the N-terminal transactivation domain, a DNA-binding domain, and a C-terminal regulatory region. Unlike its homolog Nrf2. which primarily mediates antioxidant responses, Nrf1 is essential for maintaining basal expression of proteasome subunits and redox-sensitive genes, particularly under conditions of endoplasmic reticulum (ER) stress or mitochondrial dysfunction. It is proteolytically processed in the ER or Golgi to become an active transcription factor, enabling nuclear translocation and binding to antioxidant response elements (AREs) in target gene promoters.
Antibodies targeting Nrf1 are widely used in research to investigate its expression, localization, and functional interactions in various biological contexts. These tools enable detection of Nrf1 isoforms (e.g., p120. p95. p85) through techniques like Western blotting, immunofluorescence, and immunohistochemistry. Studies utilizing Nrf1 antibodies have revealed its involvement in neurodegenerative diseases (e.g., Alzheimer’s), cancer progression, and metabolic disorders, where dysregulation of oxidative stress responses or proteasome activity occurs. However, researchers must validate antibody specificity due to potential cross-reactivity with Nrf2 or other CNC family members. Commercial Nrf1 antibodies are often raised against epitopes in the N-terminal or DNA-binding domains, with applications extending to chromatin immunoprecipitation (ChIP) for studying gene regulation mechanisms. Proper controls, including knockout cell lines or siRNA-mediated knockdown, are critical for ensuring experimental reliability.
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