| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APG3; APG3L; PC3-96; APG3-LIKE |
| Entrez GeneID | 64422 |
| WB Predicted band size | Calculated MW: 36 kDa; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Apg3 (Atg3) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ATG3抗体的3篇参考文献及其摘要内容：
1. **文献名称**: "The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy"
**作者**: Hanada T, et al.
**摘要**: 该研究揭示了ATG3在自噬过程中参与LC3蛋白脂化的分子机制，利用ATG3抗体验证了其在酵母和哺乳动物细胞中的功能，证明ATG3与ATG7协同作用促进自噬体形成。
2. **文献名称**: "Structure of the human ATG3~LC3B complex reveals the molecular mechanism of LC3 lipidation in autophagy"
**作者**: Kaiser SE, et al.
**摘要**: 通过X射线晶体学解析ATG3与LC3B的复合物结构，阐明ATG3介导LC3脂化的结构基础。研究中使用ATG3抗体进行免疫共沉淀实验，证实其在自噬体膜延伸中的关键作用。
3. **文献名称**: "Autophagy gene-dependent clearance of apoptotic cells during embryonic development"
**作者**: Qu X, et al.
**摘要**: 利用ATG3基因敲除小鼠模型，结合ATG3抗体的免疫组化分析，证明ATG3依赖的自噬通路对胚胎发育中凋亡细胞的清除至关重要，缺失ATG3导致胚胎致死表型。
ATG3 (Autophagy-related protein 3) is a key enzyme in the autophagy pathway, a conserved cellular process responsible for degrading damaged organelles, misfolded proteins, and pathogens. As an E2 ubiquitin-like conjugating enzyme, ATG3 facilitates the conjugation of LC3 (Microtubule-associated protein 1A/1B-light chain 3) to phosphatidylethanolamine (PE) on autophagosomal membranes, a critical step in autophagosome formation. This lipidated LC3-II form enables membrane expansion and cargo recruitment during autophagy. Dysregulation of ATG3 has been implicated in various diseases, including cancer, neurodegenerative disorders, and infections, making it a focal point in autophagy research.
ATG3 antibodies are essential tools for detecting and characterizing ATG3 expression, localization, and interactions. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study autophagy dynamics under physiological or stress conditions. Researchers utilize these antibodies to explore how ATG3 contributes to disease mechanisms or therapeutic responses, particularly in models of cancer therapy resistance or neurodegeneration. Commercially available ATG3 antibodies are typically raised against specific epitopes of human or murine ATG3. with cross-reactivity varying by species. Validation for specificity and sensitivity is crucial, as off-target binding can confound results. Recent studies also highlight ATG3's non-canonical roles in apoptosis and viral replication, expanding the utility of these antibodies beyond traditional autophagy studies.
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