| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | K9; CK-9; EPPK |
| Entrez GeneID | 3857 |
| WB Predicted band size | Calculated MW: 62 kDa; Observed MW: 62 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Cytokeratin 9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于Cytokeratin 9(CK9)抗体的3篇参考文献示例(基于公开信息概括，非真实文献)：
1. **文献名称**：*Cytokeratin 9 as a specific marker for epidermal differentiation in human skin*
**作者**：Smith A, et al.
**摘要**：本研究通过免疫组化验证了Cytokeratin 9抗体在人类表皮角质细胞中的特异性表达，证实其在区分表皮分化阶段中的诊断价值，尤其与掌跖部位角化相关。
2. **文献名称**：*Monoclonal antibody against Cytokeratin 9 reveals mutations in palmoplantar keratoderma*
**作者**：Lee B, et al.
**摘要**：作者开发了一种高特异性的CK9单克隆抗体，用于检测遗传性掌跖角化症患者皮肤样本中的CK9蛋白异常表达，揭示了其与KRT9基因突变的关联性。
3. **文献名称**：*Comparative analysis of Cytokeratin 9 antibody performance in epithelial tumor diagnosis*
**作者**：Zhang C, et al.
**摘要**：通过对比多种商业CK9抗体的灵敏度和特异性，本文评估了其在鳞状上皮肿瘤诊断中的应用潜力，并推荐了优化的染色条件。
(注：以上为模拟示例，实际文献需通过PubMed/Google Scholar等平台检索确认。)
Cytokeratin 9 (CK9) is a type I intermediate filament protein primarily expressed in the suprabasal layers of stratified epithelial tissues, particularly in the epidermis. As a member of the cytokeratin family, it plays a structural role in maintaining cellular integrity and mechanical resilience. CK9 is notably abundant in palmoplantar skin, where it contributes to the specialized differentiation of keratinocytes in high-friction regions. Its expression is tightly regulated during epithelial development and differentiation, serving as a marker for terminally differentiated keratinocytes.
Mutations in the *KRT9* gene, encoding CK9. are linked to epidermolytic palmoplantar keratoderma (EPPK), an autosomal dominant skin disorder characterized by hyperkeratosis and blistering on palms and soles. This association underscores CK9's critical role in epidermal stability.
CK9-specific antibodies are essential tools in research and diagnostics. They enable the identification of CK9-expressing cells in immunohistochemistry, aiding in the study of epithelial differentiation and pathological conditions like EPPK. Additionally, these antibodies help distinguish CK9 from other cytokeratins in cancer research, as cytokeratin profiles are often used to determine tumor origin. Commercial CK9 antibodies are typically validated for specificity via Western blotting or immunofluorescence, supporting applications in both basic science and clinical pathology.
×
