| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CD8A; MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD antigen CD8a |
| Entrez GeneID | 925 |
| WB Predicted band size | Calculated MW: 26 kDa; Observed MW: 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human CD8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CD8α抗体的3篇经典文献及其摘要概述：
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1. **文献名称**：*CD8α is essential for T cell development but not for T cell function*
**作者**：Robey, E., & Fowlkes, B.J.
**摘要**：该研究通过基因敲除技术揭示了CD8α在胸腺T细胞发育中的关键作用，证明CD8α缺失会阻碍MHC I限制性T细胞的成熟，但成熟后的CD8+ T细胞在免疫应答中仍保留部分功能，表明CD8α主要影响发育而非效应阶段。
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2. **文献名称**：*Structure of the human class I histocompatibility antigen, HLA-A2*
**作者**：Bjorkman, P.J., et al.
**摘要**：这篇开创性研究解析了MHC I类分子HLA-A2的晶体结构，并探讨了CD8α作为共受体通过其胞外域与MHC I结合，促进T细胞受体信号传导的分子机制，为后续CD8α抗体的功能研究奠定结构基础。
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3. **文献名称**：*Monoclonal antibodies to mouse CD8α define functional subsets*
**作者**：Ledbetter, J.A., et al.
**摘要**：研究团队开发了多种针对小鼠CD8α的单克隆抗体(如53-6.7克隆)，验证了这些抗体通过阻断CD8-MHC I互作或诱导细胞耗竭，从而在实验中调控CD8+ T细胞活性，为免疫应答机制研究提供了重要工具。
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以上文献涵盖了CD8α的发育功能、结构机制及抗体工具开发，可作为该领域的核心参考。如需具体年份或期刊细节，建议通过PubMed等数据库进一步检索。
CD8 alpha (CD8α) is a transmembrane glycoprotein expressed primarily on cytotoxic T lymphocytes (CTLs) and subsets of natural killer (NK) cells. As a member of the immunoglobulin (Ig) superfamily, CD8α forms heterodimers with CD8β or homodimers (in some dendritic cells) that function as co-receptors for major histocompatibility complex class I (MHC-I) molecules. This interaction stabilizes T cell receptor (TCR)-peptide-MHC binding and enhances antigen-specific signaling during immune synapse formation. The cytoplasmic domain of CD8α associates with the tyrosine kinase Lck, critical for initiating TCR-mediated activation cascades.
CD8 alpha-specific antibodies are vital tools in immunology research and diagnostics. They are widely used in flow cytometry to identify and isolate CD8+ T cells, which play central roles in antiviral and antitumor immunity. In immunohistochemistry, these antibodies help visualize tissue-infiltrating cytotoxic T cells. Additionally, CD8α antibodies have therapeutic applications, such as modulating T cell responses in autoimmune diseases or improving CAR-T cell targeting. Common clones like OKT8 and RPA-T8 recognize distinct epitopes of the CD8α extracellular domain, enabling versatile experimental designs. Recent studies also explore CD8α's regulatory roles beyond co-receptor function, including its involvement in T cell exhaustion and memory differentiation, highlighting the antibody's continued relevance in advancing immunotherapeutic strategies.
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