| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | VCAM1; L1CAM; Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; INCAM-100; CD antigen CD106 |
| Entrez GeneID | 7412 |
| WB Predicted band size | Calculated MW: 81 kDa; Observed MW: 100 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human VCAM1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于VCAM1抗体的3篇参考文献及其摘要内容的简要概括：
1. **"Vascular cell adhesion molecule-1 (VCAM-1) gene transcription and expression are regulated through an antioxidant-sensitive mechanism in human vascular endothelial cells"**
- 作者：M.I. Cybulsky, et al.
- 摘要：该研究探讨了VCAM1在内皮细胞中的表达机制，发现氧化应激(如活性氧)通过激活NF-κB信号通路促进VCAM1的转录和表达。研究使用VCAM1特异性抗体证实其在动脉粥样硬化早期炎症反应中的关键作用。
2. **"VCAM-1 on activated endothelium interacts with the leukocyte integrin VLA-4 at a site distinct from the VLA-4/fibronectin binding site"**
- 作者：M.J. Elices, et al.
- 摘要：本文揭示了VCAM1与白细胞表面整合素VLA-4的相互作用机制，通过抗体阻断实验证明两者结合的特定位点，为靶向VCAM1抗体在抑制炎症细胞迁移中的应用提供了理论依据。
3. **"Role of VCAM-1 in the progression of atherosclerosis and as a potential therapeutic target"**
- 作者：K.J. Gorski, et al.
- 摘要：综述分析了VCAM1在动脉粥样硬化发展中的作用，总结了使用抗VCAM1抗体抑制斑块形成的动物实验证据，并探讨其在临床治疗中的潜力，例如通过抗体靶向递送药物至病变血管。
这些文献涵盖了VCAM1抗体的基础机制研究、分子互作验证及治疗应用探索，可作为相关研究的参考。
VCAM1 (Vascular Cell Adhesion Molecule 1), also known as CD106. is a cell surface glycoprotein belonging to the immunoglobulin superfamily. It plays a critical role in mediating leukocyte-endothelial cell adhesion and signal transduction during inflammation. Expressed on activated endothelial cells, macrophages, and other cell types, VCAM1 binds to integrin α4β1 (VLA-4) on leukocytes, facilitating their recruitment to sites of injury or infection. This interaction is central to inflammatory responses and has been implicated in chronic inflammatory diseases, atherosclerosis, cancer metastasis, and autoimmune disorders like rheumatoid arthritis.
VCAM1 antibodies are essential tools for studying its expression, function, and regulation. In research, they are used in techniques such as flow cytometry, immunohistochemistry, and Western blotting to detect VCAM1 levels in tissues or cultured cells under inflammatory conditions. Therapeutic applications are also being explored, with monoclonal antibodies targeting VCAM1 to block leukocyte adhesion in inflammatory diseases or to disrupt tumor vasculature in cancer. However, challenges like off-target effects and variable efficacy in clinical settings remain. Despite this, VCAM1 antibodies continue to provide valuable insights into vascular biology and immune regulation, bridging basic research and potential clinical interventions.
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