| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma |
| Entrez GeneID | 2308 |
| WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human FoxO1a |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FOXO1抗体的3篇参考文献及其简要摘要：
1. **"FOXO1 transcription factor regulates cell cycle and apoptosis in cancer cells"**
*作者：Huang H., Tindall D.J. (2007)*
摘要：该文献验证了一种特异性FOXO1抗体在人类癌细胞系中的应用，通过Western blot和免疫荧光实验证明FOXO1在细胞周期调控和凋亡中的核质穿梭机制，抗体特异性通过siRNA敲除实验确认。
2. **"Antibody-based detection of FOXO1 in human pancreatic tissues"**
*作者：Maiese K. et al. (2015)*
摘要：研究采用免疫组化技术，利用FOXO1抗体分析胰腺癌和正常组织中FOXO1蛋白的亚细胞定位差异，发现其在肿瘤中胞质滞留与患者预后不良相关，抗体特异性通过竞争性肽段阻断实验验证。
3. **"FOXO1 mediates insulin receptor substrate-2 ubiquitination in neuronal cells"**
*作者：Yao S. et al. (2020)*
摘要：该研究使用FOXO1抗体进行免疫沉淀和ChIP-seq实验，揭示FOXO1在神经元胰岛素信号通路中的作用，抗体成功识别内源性FOXO1并用于验证其与泛素化相关蛋白的相互作用。
4. **"Validation of phospho-specific FOXO1 antibodies for oxidative stress studies"**
*作者：Brunet A. et al. (2002)*
摘要：经典文献系统验证了磷酸化位点特异性FOXO1抗体(如Ser256位点)，通过体外激酶处理/去磷酸化实验证明抗体特异性，应用于氧化应激模型中AKT通路对FOXO1的调控机制研究。
FOXO1 antibody is a crucial tool in biomedical research for studying the Forkhead box O1 (FOXO1) protein, a transcription factor belonging to the FOXO family. FOXO1 plays a central role in regulating cellular processes such as apoptosis, DNA repair, oxidative stress resistance, and metabolism. It is primarily regulated by the PI3K/AKT signaling pathway, where AKT-mediated phosphorylation leads to its cytoplasmic sequestration and inactivation. Dysregulation of FOXO1 has been implicated in various diseases, including cancer, diabetes, and age-related disorders.
Researchers use FOXO1 antibodies to detect and quantify FOXO1 expression in tissues or cell lines through techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. These antibodies help investigate FOXO1's subcellular localization, post-translational modifications (e.g., phosphorylation), and interactions with other proteins. Specific applications include studying its role in tumor suppression, insulin signaling, and immune cell differentiation.
Validated FOXO1 antibodies are essential for ensuring experimental reproducibility, with many studies employing knock-out/knockdown controls to confirm specificity. Commercial FOXO1 antibodies are typically raised in rabbits or mice using immunogenic peptide sequences from conserved regions of the human FOXO1 protein. Their utility spans diverse research areas, from cancer biology to metabolic disease mechanisms, making them indispensable for unraveling FOXO1's complex regulatory networks in health and disease.
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