| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ALDH1A1; ALDC; ALDH1; PUMB1; Retinal dehydrogenase 1; RALDH 1; RalDH1; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase; cytosolic |
| Entrez GeneID | 216 |
| WB Predicted band size | Calculated MW: 55 kDa; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ALDH1A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于ALDH1A1抗体的参考文献示例(内容基于公开研究整理，具体引用请核实原文)：
1. **文献名称**：*ALDH1A1 is a marker of malignant prostate stem cells and a predictor of prostate cancer progression*
**作者**：Marcato P 等
**摘要**：研究使用ALDH1A1抗体通过免疫组化分析前列腺癌组织，发现ALDH1A1高表达与癌症干细胞特性相关，且与肿瘤转移和不良预后显著关联。
2. **文献名称**：*ALDH1A1-mediated retinoic acid signaling is required for stem cell maintenance in head and neck squamous cell carcinoma*
**作者**：Chen Y 等
**摘要**：通过ALDH1A1抗体流式分选头颈鳞癌细胞，证实ALDH1A1+细胞具有自我更新能力，其表达依赖视黄酸信号通路，靶向该通路可抑制肿瘤生长。
3. **文献名称**：*ALDH1A1-positive cancer stem cells predict chemoresistance in ovarian cancer*
**作者**：Deng S 等
**摘要**：采用ALDH1A1抗体检测卵巢癌细胞系及患者样本，发现ALDH1A1+细胞对顺铂耐药，抑制ALDH1活性可增强化疗敏感性。
4. **文献名称**：*Comparison of ALDH1A1 antibody-based detection versus enzymatic activity assay in solid tumors*
**作者**：Sládek NE 等
**摘要**：研究对比了ALDH1A1抗体(免疫荧光)与酶活性检测(ALDEFLUOR试剂)在实体瘤中的一致性，发现两者在识别干细胞亚群时具有互补性，需结合使用以提高准确性。
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**注意**：以上为模拟摘要，实际文献需通过PubMed、Web of Science等平台检索。推荐使用关键词“ALDH1A1 antibody” + “cancer stem cells”/“chemoresistance”等进一步筛选。
**Background of ALDH1A1 Antibody**
ALDH1A1 (Aldehyde Dehydrogenase 1 Family Member A1) is a cytosolic enzyme belonging to the aldehyde dehydrogenase superfamily, which catalyzes the oxidation of aldehydes to carboxylic acids. It plays a critical role in cellular detoxification, stem cell maintenance, and retinoic acid biosynthesis by converting retinaldehyde to retinoic acid, a key signaling molecule in development and differentiation. ALDH1A1 is widely studied in cancer biology due to its overexpression in cancer stem cells (CSCs), where it contributes to chemoresistance, tumor progression, and poor prognosis in cancers such as breast, ovarian, and lung malignancies.
ALDH1A1-specific antibodies are essential tools for detecting and quantifying ALDH1A1 protein expression in research and clinical samples. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to study tissue distribution, subcellular localization, and expression levels in normal versus pathological states. Monoclonal antibodies offer high specificity, whereas polyclonal antibodies may detect multiple epitopes, enhancing sensitivity. Validation of ALDH1A1 antibodies typically includes testing in knockout cell lines or tissues to confirm target specificity.
Research applications span cancer biomarker discovery, stem cell biology, and metabolic disease studies. In diagnostics, ALDH1A1 expression is evaluated as a potential prognostic indicator or therapeutic target. Commercial ALDH1A1 antibodies are often optimized for compatibility with formalin-fixed, paraffin-embedded samples, ensuring utility in archival clinical research. Proper controls and standardized protocols are critical to minimize variability in experimental outcomes.
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