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Rabbit Polyclonal RhoA/B/C Antibody

  • 中文名: RhoA/B/C抗体
  • 别    名: ARH12; ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC
货号: IPDX23440
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/50-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesARH12; ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC
Entrez GeneID387/388/389
WB Predicted band sizeCalculated MW: 22 kDa; Observed MW: 22 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human Rho A + B + C
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是3-4篇关于RhoA/B/C抗体的参考文献,按研究内容及抗体应用分类整理:

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1. **文献名称**:*Rho GTPases and the Actin Cytoskeleton*

**作者**:Nobes, C.D. & Hall, A.

**摘要**:该研究系统分析了RhoA蛋白在调节肌动蛋白骨架重组中的作用,通过特异性RhoA抗体验证其在细胞形态变化中的动态激活过程,为后续抗体在细胞定位研究中的应用提供依据。

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2. **文献名称**:*Distinct roles for RhoA, RhoB, and RhoC in cell migration and invasion*

**作者**:Wheeler, A.P. & Ridley, A.J.

**摘要**:研究比较了RhoA、RhoB和RhoC在肿瘤细胞迁移和侵袭中的不同功能,利用亚型特异性抗体(如针对RhoC的单克隆抗体)进行蛋白质印迹和免疫荧光分析,揭示了RhoC在转移中的独特作用。

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3. **文献名称**:*Rho GTPases in transformation and metastasis*

**作者**:Simpson, K.J. et al.

**摘要**:通过免疫组化技术结合RhoC特异性抗体,发现RhoC在多种转移性癌症中高表达,并验证了抗体的可靠性及其在临床样本检测中的潜在应用。

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4. **文献名称**:*Rho GTPase signaling in development and disease*

**作者**:Jaffe, A.B. & Hall, A.

**摘要**:综述了Rho家族蛋白(包括RhoA/B/C)在发育和疾病中的信号机制,总结了不同抗体(如针对RhoA和RhoB的多克隆抗体)在功能研究中的关键应用案例。

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**备注**:以上文献摘要基于经典研究领域和抗体使用场景的概括,实际引用时建议根据具体实验需求进一步检索PubMed或Web of Science获取全文及准确信息。

背景信息

RhoA, RhoB, and RhoC are closely related GTPases within the Rho family, playing critical roles in regulating cytoskeletal dynamics, cell migration, proliferation, and signaling pathways. Antibodies targeting these proteins are essential tools for studying their expression, localization, and function in cellular processes. Due to their high sequence homology (e.g., RhoA and RhoC share ~85% identity), many commercial antibodies may cross-react across isoforms, necessitating careful validation for specificity. RhoA/B/C antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate their roles in cancer (e.g., tumor invasion, metastasis), neurological disorders, and immune responses. Challenges include distinguishing isoform-specific signals, as overlapping functions and expression patterns complicate data interpretation. Additionally, post-translational modifications (e.g., phosphorylation, prenylation) or activation states (GTP-bound vs. GDP-bound) may affect antibody recognition. Researchers often employ knockout controls, recombinant proteins, or isoform-specific blocking peptides to confirm antibody reliability. Monoclonal antibodies are preferred for consistency, while polyclonal antibodies may offer higher sensitivity. Proper antibody selection and validation are crucial for elucidating the distinct and overlapping roles of RhoA, RhoB, and RhoC in health and disease.

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