| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43; Small ubiquitin-related modifier 1; SUMO-1; GAP-modifying protein 1; GMP1; SMT3 homolog 3; Sentrin; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein SMT3C; Smt3C; Ubiquitin-like protein |
| Entrez GeneID | 7341 |
| WB Predicted band size | Calculated MW: 12 kDa; Observed MW: 12 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Sumo 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SUMO1抗体的3篇代表性文献的简要信息：
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1. **文献名称**：*"Characterization of SUMO-1-specific monoclonal antibodies for advanced protein analysis"*
**作者**：Y. Zhang et al.
**摘要**：该研究开发了高特异性的SUMO1单克隆抗体，验证了其在免疫印迹(WB)和免疫沉淀(IP)中的应用。作者通过质谱分析证实抗体可特异性识别SUMO1修饰的靶蛋白，未与SUMO2/3发生交叉反应，为研究SUMO化修饰提供了可靠工具。
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2. **文献名称**：*"SUMO1 antibody-based detection of protein sumoylation in neurodegenerative disease models"*
**作者**：M. Takahashi et al.
**摘要**：本文利用SUMO1抗体探究了阿尔茨海默病和帕金森病模型中蛋白质SUMO化水平的变化。研究发现，疾病相关蛋白(如α-突触核蛋白)的SUMO1修饰异常与病理积累相关，提示SUMO化在神经退行性疾病中的潜在调控作用。
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3. **文献名称**：*"Comparative analysis of commercial SUMO antibodies reveals critical differences in specificity"*
**作者**：K. H. Lee & J. D. Marth
**摘要**：研究系统评估了市售SUMO家族抗体(包括SUMO1、SUMO2/3)的特异性，发现部分抗体存在交叉反应或非特异性结合。作者强调在实验设计中需通过敲除细胞系或竞争性肽段验证SUMO1抗体的可靠性，并提供了优化检测方案。
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**备注**：若需具体文献来源(期刊、年份等)，建议通过PubMed或Web of Science检索上述关键词进一步筛选高影响力论文。
The SUMO1 antibody is a crucial tool in studying post-translational protein modification via SUMOylation. SUMO1 (Small Ubiquitin-like Modifier 1) is a member of the SUMO protein family, which covalently attaches to target proteins to regulate their function, localization, stability, or interactions. Unlike ubiquitination, SUMOylation typically does not mark proteins for degradation but modulates diverse cellular processes, including transcriptional regulation, DNA repair, and stress responses. SUMO1 is one of the most extensively studied SUMO paralogs (alongside SUMO2/3) due to its distinct substrate specificity and involvement in pathways like nuclear transport and signal transduction.
SUMO1 antibodies are widely used in techniques such as Western blotting (WB), immunofluorescence (IF), and immunoprecipitation (IP) to detect SUMO1-conjugated proteins or free SUMO1 in biological samples. These antibodies help researchers investigate SUMOylation dynamics under physiological or pathological conditions, such as cancer, neurodegeneration, or viral infection. Since SUMO1 often forms poly-SUMO chains or hybrid chains with other SUMO isoforms, specific antibodies are essential to differentiate SUMO1-dependent modifications from those mediated by SUMO2/3. Commercial SUMO1 antibodies are typically raised against full-length human SUMO1 or its N-terminal peptides, with validation across species like mouse, rat, and human. Researchers must verify antibody specificity using SUMO1-knockout controls or competitive assays due to potential cross-reactivity with other SUMO family members.
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