| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AURKB; AIK2; AIM1; AIRK2; ARK2; STK1; STK12; STK5; Aurora kinase B; Aurora 1; Aurora- and IPL1-like midbody-associated protein 1; AIM-1; Aurora/IPL1-related kinase 2; ARK-2; Aurora-related kinase 2; STK-1; Serine/threonine-protein kinase 12 |
| Entrez GeneID | 9212 |
| WB Predicted band size | Calculated MW: 39 kDa; Observed MW: 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Aurora B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Aurora B kinase is recruited to centromeres by the chromosomal passenger complex and regulates chromosome segregation"**
*作者：Carmena M.等*
摘要：研究Aurora B在染色体分离中的作用，揭示其通过染色体乘客复合体(CPC)定位至着丝粒，调控纺锤体微管附着和错误纠正的机制。
2. **"Structure and function of Aurora B–INCENP complex in mitosis"**
*作者：Sessa F.等*
摘要：解析Aurora B与INCENP蛋白复合物的结构，阐述其磷酸化底物及在胞质分裂中的活性调控机制，为抗体靶向设计提供依据。
3. **"Aurora B kinase as a therapeutic target in cancer"**
*作者：Katayama H.等*
摘要：综述Aurora B在肿瘤中的过表达现象，讨论其抗体及小分子抑制剂在癌症治疗中的潜力，并总结相关临床试验结果。
4. **"Dynamic localization of Aurora B kinase during meiosis"**
*作者：Yoon HJ.等*
摘要：利用特异性抗体追踪Aurora B在减数分裂中的动态定位，揭示其在同源染色体分离和姐妹染色单体黏连中的作用差异。
Aurora B kinase antibody is a research tool designed to detect Aurora B, a critical serine/threonine kinase involved in regulating mitosis. Aurora B, part of the chromosomal passenger complex (CPC), ensures accurate cell division by monitoring chromosome alignment, correcting microtubule-kinetochore attachment errors, and facilitating cytokinesis. Dysregulation of Aurora B is linked to genomic instability, aneuploidy, and cancer progression, making it a prominent target in oncology research.
Aurora B antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study its expression, localization, and activity in cell lines, tissues, or tumor samples. These antibodies help investigate Aurora B's role in cell cycle checkpoints, its overexpression in cancers (e.g., breast, colon, and lung), and its potential as a therapeutic target. Specific clones or epitope-targeted antibodies may vary in sensitivity and applications, so validation using knockout controls or pharmacological inhibitors (e.g., ZM447439) is essential.
Researchers also employ Aurora B antibodies to explore its crosstalk with other mitotic regulators (e.g., Survivin, INCENP) and evaluate inhibitors in preclinical models. As Aurora B inhibitors enter clinical trials, these antibodies remain vital for mechanistic studies and biomarker analysis in cancer therapy development.
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