| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase LATS1 (EC 2.7.11.1; Large tumor suppressor homolog 1; WARTS protein kinase; h-warts) |
| Entrez GeneID | 9113 |
| WB Predicted band size | Calculated MW: 127 kDa; Observed MW: 120 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human LATS1/2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于LATS1抗体的3篇参考文献示例(注：内容为虚构，仅用于示例格式)：
1. **文献名称**：LATS1 Phosphorylation Status in Breast Cancer Progression
**作者**：Smith A, et al.
**摘要**：本研究利用特异性LATS1抗体检测乳腺癌组织中LATS1的磷酸化水平，发现其失活与肿瘤转移相关，提示LATS1可能作为预后标志物。
2. **文献名称**：LATS1 Interaction with YAP in Hippo Signaling
**作者**：Chen L, et al.
**摘要**：通过免疫共沉淀(Co-IP)结合LATS1抗体，证实LATS1与YAP蛋白的直接相互作用，揭示了Hippo通路调控细胞增殖的分子机制。
3. **文献名称**：Development of a Novel Monoclonal Antibody for LATS1 Detection
**作者**：Wang X, et al.
**摘要**：报道一种高特异性小鼠源LATS1单克隆抗体的开发，验证其在Western blot和免疫荧光中的应用，为Hippo通路研究提供新工具。
(提示：实际文献需通过PubMed/Google Scholar检索关键词“LATS1 antibody”获取。)
The LATS1 (Large Tumor Suppressor Kinase 1) antibody is a crucial tool in studying the Hippo signaling pathway, a conserved regulator of organ size, tissue homeostasis, and tumor suppression. LATS1. a serine/threonine kinase, acts as a central effector of this pathway. It phosphorylates and inhibits YAP/TAZ transcriptional coactivators, preventing their nuclear translocation and pro-growth signaling. Dysregulation of LATS1 is linked to cancer, fibrosis, and developmental disorders.
LATS1 antibodies are widely used to detect protein expression, phosphorylation status, and subcellular localization in various models (cells, tissues, or organisms). They enable investigations into Hippo pathway activity, particularly in contexts like tumorigenesis, cell proliferation, apoptosis, and mechanotransduction. Common applications include Western blotting, immunofluorescence, immunohistochemistry, and co-immunoprecipitation assays.
Commercially available antibodies target specific epitopes, including full-length proteins or phosphorylated forms (e.g., p-LATS1 at Thr1079). Researchers must validate antibody specificity using knockout controls or siRNA, as cross-reactivity with LATS2 (a paralog sharing ~50% homology) may occur. Batch variability and species reactivity (human, mouse, rat) should also be considered. These antibodies have advanced studies in cancer biology, tissue regeneration, and developmental processes, highlighting LATS1's role as a tumor suppressor and cellular growth modulator.
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