| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Complement component C8 alpha chain (Complement component 8 subunit alpha) |
| Entrez GeneID | 731 |
| WB Predicted band size | Calculated MW: 65 kDa; Observed MW: 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from human C8 α. at AA range: 201-250 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于C8 alpha抗体的3篇代表性文献摘要(内容为模拟示例，具体文献需通过学术数据库验证)：
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1. **文献名称**: *Structural characterization of the human complement C8α chain and its recognition by autoantibodies in autoimmune disorders*
**作者**: Smith A, et al.
**摘要**: 本研究解析了C8α链的晶体结构，并发现其在膜攻击复合物(MAC)组装中的关键作用。通过ELISA和免疫印迹实验，发现系统性红斑狼疮(SLE)患者血清中的自身抗体能特异性识别C8α的特定表位，提示其与疾病病理相关。
2. **文献名称**: *C8α antibody inhibits complement-mediated lysis in paroxysmal nocturnal hemoglobinuria (PNH) models*
**作者**: Brown L, et al.
**摘要**: 开发了一种针对C8α的单克隆抗体，证明其可通过阻断MAC形成保护PNH患者的红细胞免受补体攻击。体外实验显示该抗体显著降低溶血率，为靶向补体终末阶段的治疗策略提供依据。
3. **文献名称**: *Functional mapping of C8α epitopes using monoclonal antibodies*
**作者**: Zhang Y, et al.
**摘要**: 利用单克隆抗体技术鉴定了C8α蛋白的功能性表位，发现其MAC组装相关结构域的抗原性区域。研究揭示了抗体结合对C9聚合的抑制作用，为补体调控药物设计提供新靶点。
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如需具体文献，建议在PubMed或Google Scholar中检索关键词“C8 alpha antibody complement”或“anti-C8α autoantibodies”获取最新研究。
**Background of C8 Alpha Antibody**
The C8 alpha antibody targets the alpha subunit of complement component 8 (C8α), a critical protein in the complement system, an innate immune defense mechanism. C8 is part of the membrane attack complex (MAC), which forms pores in pathogen membranes to lyse microbes. C8 itself is a heterotrimer composed of alpha, beta, and gamma subunits. The C8α subunit, encoded by the *C8A* gene, binds to C5b-7 complexes during MAC assembly, facilitating the polymerization of C9 to complete the pore structure.
C8α plays a dual role: promoting pathogen clearance and contributing to inflammatory tissue damage in autoimmune or hyperinflammatory conditions. Deficiencies in C8α are linked to increased susceptibility to bacterial infections, particularly *Neisseria* species. Research on C8α antibodies focuses on understanding MAC dynamics, diagnosing complement-related disorders, and exploring therapeutic interventions. For instance, antibodies against C8α are used to study MAC formation in diseases like atypical hemolytic uremic syndrome (aHUS) or age-related macular degeneration (AMD), where dysregulated complement activation drives pathology.
Additionally, C8α antibodies serve as tools in immunodetection assays (e.g., Western blot, immunofluorescence) to analyze protein expression or localization. Recent studies also investigate blocking C8α to modulate MAC activity, potentially mitigating complement-mediated tissue injury. Overall, C8α antibodies are pivotal in both basic research and clinical applications related to complement-mediated immunity and disease.
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