| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Collagen alpha-2(VIII) chain (Endothelial collagen) |
| Entrez GeneID | 1296 |
| WB Predicted band size | Calculated MW: 67 kDa; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human COL8A2. at AA range: 611-660 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是3条与Collagen VIII alpha 2(COL8A2)抗体相关的研究文献示例(内容基于公开研究领域知识概括，非真实文献)：
1. **文献名称**：*"COL8A2 mutations impair extracellular matrix deposition and cause posterior polymorphous corneal dystrophy"*
**作者**：Biswas S, et al.
**摘要**：研究通过免疫组化及COL8A2抗体检测，发现COL8A2基因突变导致角膜内皮细胞外基质异常沉积，与后部多形性角膜营养不良(PPCD)的病理机制相关。
2. **文献名称**：*"Role of Collagen VIII in angiogenesis: Insights from COL8A2 knockout mice"*
**作者**：Park JH, Ahn Y.
**摘要**：利用COL8A2抗体标记血管内皮细胞，揭示COL8A2通过调控VEGF信号通路促进血管生成，基因敲除小鼠模型显示血管发育缺陷。
3. **文献名称**：*"COL8A2 overexpression in tumor-associated fibroblasts promotes cancer invasion"*
**作者**：Chen L, et al.
**摘要**：通过免疫荧光和Western blot分析，发现肿瘤微环境中COL8A2高表达的成纤维细胞通过激活MMP2促进癌细胞侵袭，抗体阻断实验验证其功能。
4. **文献名称**：*"Autoantibodies against COL8A2 as a biomarker in diabetic retinopathy"*
**作者**：Noda K, et al.
**摘要**：研究报道糖尿病患者血清中抗COL8A2抗体水平升高，可能与视网膜血管基底膜损伤相关，提示其作为疾病进展的生物标志物潜力。
(注：以上为模拟文献，实际引用需查询PubMed等数据库获取真实信息。)
Collagen VIII alpha 2 (COL8A2) is a component of type VIII collagen, a short-chain collagen primarily found in basement membranes, particularly around vascular endothelial and epithelial cells. Encoded by the COL8A2 gene, this protein forms heterotrimers with the alpha 1 chain (COL8A1) to create a hexagonal network structure that supports extracellular matrix organization. Type VIII collagen plays a role in regulating cell adhesion, migration, and tissue remodeling, with implications in angiogenesis, wound healing, and maintaining tissue integrity.
Antibodies targeting COL8A2 are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect COL8A2 in cell cultures, tissue sections, or biological fluids. These antibodies have been particularly valuable in researching corneal dystrophies, such as posterior polymorphous corneal dystrophy (PPCD1), which is linked to COL8A2 mutations. Additionally, they contribute to investigations into vascular disorders, fibrosis, and tumor microenvironment dynamics, where altered collagen VIII expression is observed.
Commercial COL8A2 antibodies are available as polyclonal or monoclonal forms, often validated for specificity using knockout controls or siRNA-based silencing. Understanding COL8A2’s role through these reagents has advanced insights into its dual functionality as a structural protein and a modulator of cellular signaling pathways, highlighting its potential as a therapeutic or diagnostic target in connective tissue diseases.
×
