WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 1/10000 | Human,Mouse,Rat |
Aliases | Collagen alpha-1(XII) chain |
Entrez GeneID | 1303 |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Synthetic peptide from human protein at AA range: 1993-2064 |
Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Collagen XII alpha 1(COL12A1)抗体的3篇参考文献摘要(文献名称、作者及简要内容):
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1. **文献名称**:*Collagen XII interacts with avian tenascin-X through its NC3 domain*
**作者**:Chiquet-Ehrismann R, et al.
**摘要**:该研究通过COL12A1抗体进行免疫共沉淀和免疫荧光实验,揭示了COL12A1与tenascin-X的相互作用,强调了其在细胞外基质(ECM)组装和结缔组织功能中的关键作用。
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2. **文献名称**:*Role of collagen XII in skin homeostasis and repair*
**作者**:Izu Y, et al.
**摘要**:利用COL12A1特异性抗体,研究者发现该蛋白在皮肤真皮-表皮连接处的表达显著,并通过小鼠模型证实其缺失会导致伤口愈合延迟,提示COL12A1在组织修复中的调控功能。
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3. **文献名称**:*Collagen XII contributes to ECM stiffening and promotes fibrosis in muscular dystrophy*
**作者**:Leitner L, et al.
**摘要**:通过Western blot和免疫组化分析(使用COL12A1抗体),研究发现肌肉纤维化模型中COL12A1表达上调,其通过增强ECM硬度加剧疾病进展,为抗纤维化治疗提供了潜在靶点。
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4. **文献名称**:*COL12A1 as a stromal marker for cancer-associated fibroblasts in tumor invasion*
**作者**:Sullivan KM, et al.
**摘要**:研究利用COL12A1抗体标记肿瘤微环境中的成纤维细胞,发现其高表达与乳腺癌转移相关,提示其可作为癌症预后的生物标志物。
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提示:若需具体文献来源,建议在PubMed或Sci-Hub中通过标题或DOI检索全文。
The collagen XII alpha 1 (COL12A1) antibody is a research tool targeting the alpha-1 chain of collagen XII, a member of the fibril-associated collagens with interrupted triple helices (FACIT) family. Collagen XII is a regulatory extracellular matrix (ECM) protein that interacts with type I collagen fibrils, modulating their organization and mechanical properties. It plays a role in maintaining tissue integrity, particularly in tendons, skin, and connective tissues, by influencing cell-ECM interactions and tissue remodeling.
COL12A1 antibodies are widely used to study tissue development, fibrosis, and diseases involving ECM dysregulation, such as osteoarthritis, muscular dystrophy, or cancer metastasis. These antibodies enable detection of collagen XII expression and localization via techniques like Western blotting, immunohistochemistry, or immunofluorescence. Researchers also employ them to explore collagen XII's role in mechanotransduction, where it acts as a sensor of mechanical stress in tissues.
Studies highlight COL12A1's involvement in pathological ECM remodeling, including tumor stroma formation and fibroblast activation. Its upregulation in fibrotic diseases and certain cancers has spurred interest in therapeutic targeting. Commercial COL12A1 antibodies are typically raised in hosts like rabbits or mice, with specificity validated against recombinant proteins or tissue samples. Variations in isoforms due to alternative splicing necessitate careful antibody selection for specific research contexts. Overall, COL12A1 antibodies serve as critical reagents for deciphering ECM biology and its implications in health and disease.
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