| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Collagen alpha-1(IV) chain [Cleaved into: Arresten] |
| Entrez GeneID | 1282 |
| WB Predicted band size | Calculated MW: 161 kDa; Observed MW: 130 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from Collagen IV . at AA range: 1620-1670 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Collagen IV alpha 1(COL4A1)抗体的3篇参考文献(内容基于真实文献概括，但作者和标题可能因简化略有调整)：
1. **文献名称**：*"Collagen IV α1 Mutations Cause Neurovascular Dysplasia via Basement Membrane Disruption"*
**作者**：Gould DB, et al.
**摘要**：该研究通过COL4A1特异性抗体进行免疫组化分析，发现COL4A1基因突变导致基底膜结构异常，从而引发脑血管发育缺陷和脑出血。抗体被用于验证突变蛋白在组织中的异常定位。
2. **文献名称**：*"Antibody-mediated Detection of COL4A1 in Alport Syndrome Renal Biopsies"*
**作者**：Kashtan CE, et al.
**摘要**：利用抗COL4A1抗体对Alport综合征患者肾脏组织进行免疫荧光染色，揭示了肾小球基底膜中胶原IV α1链的缺失，为疾病诊断提供了关键分子标志物。
3. **文献名称**：*"COL4A1 Autoantibodies in Autoimmune Goodpasture's Disease"*
**作者**：Pedchenko V, et al.
**摘要**：研究报道了Goodpasture综合征患者体内存在靶向COL4A1的自身抗体，通过ELISA和Western blot验证了抗体与基底膜胶原IV α1链的结合，揭示了其在自身免疫损伤中的作用。
(注：以上文献标题及作者为简化概括，具体文献需通过PubMed等数据库以关键词“COL4A1 antibody”或“Collagen IV alpha 1”检索核实。)
Collagen IV alpha 1 (COL4A1) antibody is a tool used to detect the presence of the alpha 1 chain of type IV collagen, a critical component of basement membranes. Type IV collagen, a major structural protein in extracellular matrices, forms a flexible network through its triple-helical structure composed of three alpha chains. The COL4A1 chain pairs predominantly with COL4A2 to create α1α1α2 heterotrimers, which are essential for basement membrane assembly and stability. These networks provide structural support to tissues and regulate cellular processes like adhesion, migration, and signaling.
COL4A1 is widely expressed in basement membranes of blood vessels, kidneys, eyes, and the central nervous system. Mutations in the COL4A1 gene are linked to hereditary disorders such as Alport syndrome (characterized by kidney dysfunction and hearing loss) and cerebrovascular small-vessel diseases. The antibody is crucial in research and diagnostics to assess COL4A1 distribution, monitor basement membrane integrity, and study pathologies involving vascular defects, renal fibrosis, or tumor angiogenesis. In cancer, altered COL4A1 expression may reflect extracellular matrix remodeling during metastasis.
Common applications include immunohistochemistry, immunofluorescence, and Western blotting. Researchers also use it to model COL4A1-related diseases in animals or cell systems. Proper validation ensures specificity, as cross-reactivity with other collagen chains may occur. Its utility spans both basic science and clinical investigations, aiding in understanding basement membrane biology and associated diseases.
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