| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PODXL2; Podocalyxin-like protein 2; Endoglycan |
| Entrez GeneID | 50512 |
| WB Predicted band size | Calculated MW: 65 kDa; Observed MW: 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from the Internal region of human Endoglycan. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Endoglycan抗体的3篇虚构参考文献示例(仅供格式参考，非真实文献)：
1. **文献名称**：*Endoglycan: A Novel Selectin Ligand in Leukocyte Trafficking*
**作者**：Müller, C. et al.
**摘要**：研究揭示了Endoglycan作为选择素(selectin)家族的新型配体，参与白细胞与内皮细胞的粘附过程。作者开发了一种特异性Endoglycan单克隆抗体，证实其在体外可抑制炎症模型中白细胞的滚动迁移，提示其在炎症性疾病中的潜在治疗价值。
2. **文献名称**：*Endoglycan Expression in Tumor Vasculature: A Biomarker for Cancer Progression*
**作者**：Tanaka, H. et al.
**摘要**：通过Endoglycan抗体免疫组化分析，研究发现Endoglycan在多种实体瘤(如乳腺癌和胶质瘤)的血管内皮中高表达，且与肿瘤转移相关。该抗体可用于评估患者预后及靶向药物开发。
3. **文献名称**：*Characterization of Endoglycan-Specific Antibodies for Flow Cytometry Applications*
**作者**：Lee, S. & Patel, R.
**摘要**：本文报道了两种高亲和力Endoglycan抗体的开发与验证，适用于流式细胞术检测造血干细胞表面Endoglycan的表达。研究证明抗体在急性髓系白血病(AML)患者的细胞分型中具有诊断潜力。
(注：以上内容为模拟示例，实际文献需通过学术数据库检索。)
Endoglycan is a transmembrane sialomucin protein belonging to the CD34 family, which includes other glycoproteins like CD34 and podocalyxin. It is primarily expressed on vascular endothelium, leukocytes, and certain stem cells. Structurally, Endoglycan contains heavily glycosylated extracellular domains that mediate cell-cell interactions and modulate cellular adhesion processes. Its anti-adhesive properties are attributed to the negatively charged sialic acid residues, which create repulsive forces between cells and the extracellular matrix.
Antibodies targeting Endoglycan have become valuable tools in studying vascular biology, immune cell trafficking, and tumor microenvironment dynamics. These antibodies are commonly used in flow cytometry, immunohistochemistry, and Western blotting to identify Endoglycan-expressing cells in tissues or experimental models. Research suggests Endoglycan plays roles in leukocyte extravasation during inflammation, endothelial barrier regulation, and cancer metastasis by influencing cell motility. Its expression patterns in tumor vasculature have also sparked interest in potential diagnostic or therapeutic applications. Commercially available monoclonal antibodies are typically raised against specific epitopes in its extracellular mucin domain. Recent studies utilize these reagents to explore Endoglycan's interaction with L-selectin and other adhesion molecules, highlighting its dual role in both inhibiting and facilitating cellular interactions depending on biological context.
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