| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | EDN1; Endothelin-1; Preproendothelin-1; PPET1 |
| Entrez GeneID | 1906 |
| WB Predicted band size | Calculated MW: 24 kDa; Observed MW: 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human EDN1. AA range:131-180 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Endothelin 1(ET-1)抗体的3篇文献摘要概览：
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1. **文献名称**：*Production and characterization of monoclonal antibodies against endothelin-1*
**作者**：Yanagisawa M., et al.
**摘要**：该研究报道了针对Endothelin 1的单克隆抗体的开发与特性分析。作者通过免疫小鼠获得抗体，验证了其高特异性和亲和力，并成功应用于免疫组织化学和ELISA检测，为后续ET-1的病理生理研究提供工具。
2. **文献名称**：*Endothelin-1 antibody-based detection of vascular dysfunction in hypertensive models*
**作者**：Barton M., et al.
**摘要**：研究利用ET-1特异性抗体，通过免疫荧光和Western blot技术，揭示了高血压动物模型中内皮素-1在血管壁的过度表达及其与血管收缩、炎症的关系，为心血管疾病的机制研究提供依据。
3. **文献名称**：*Endothelin-1 as a biomarker in cancer: Immunohistochemical analysis using a novel antibody*
**作者**：Grant K., et al.
**摘要**：本文开发了一种新型ET-1抗体，用于检测多种癌症组织中ET-1的表达水平。结果显示，ET-1在肿瘤血管内皮细胞和部分癌细胞中高表达，提示其可能作为癌症进展和预后的潜在标志物。
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以上文献涵盖ET-1抗体的开发、验证及在心血管疾病和癌症领域的应用。如需具体期刊信息或发表年份，可进一步补充关键词查询。
Endothelin-1 (ET-1) is a potent vasoconstrictive peptide primarily produced by vascular endothelial cells, belonging to the endothelin family. It plays a critical role in regulating vascular tone, cell proliferation, and inflammation, with dysregulation linked to cardiovascular diseases, pulmonary hypertension, and chronic kidney disease. ET-1 is synthesized as a preprohormone (preproendothelin) that undergoes proteolytic processing to generate the mature 21-amino-acid peptide. Its activity is mediated via binding to endothelin receptors (ETA and ETB), triggering signaling pathways involved in vasoconstriction and fibrosis.
ET-1 antibodies are essential tools for detecting and quantifying ET-1 expression in research and diagnostics. These antibodies target specific epitopes of the peptide, enabling applications such as immunohistochemistry, Western blotting, and ELISA. They are widely used to study ET-1's pathological roles, including its contribution to endothelial dysfunction, hypertension, and cancer progression. Polyclonal and monoclonal antibodies vary in specificity; some recognize mature ET-1. while others detect precursor forms (e.g., big ET-1). Validation of antibody specificity is crucial, as cross-reactivity with related endothelins (ET-2. ET-3) can compromise data accuracy.
Research utilizing ET-1 antibodies has advanced therapeutic developments, such as endothelin receptor antagonists (e.g., bosentan) for pulmonary arterial hypertension. These tools continue to elucidate ET-1's complex interactions in disease mechanisms and potential biomarkers for clinical monitoring.
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