| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | RASSF8; C12orf2; Ras association domain-containing protein 8; Carcinoma-associated protein HOJ-1 |
| Entrez GeneID | 11228 |
| WB Predicted band size | Calculated MW: 48 kDa; Observed MW: 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Synthesized peptide derived from RASSF8 . at AA range: 60-140 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于RASSF8抗体的3篇参考文献的简要信息(注：部分文献为模拟示例，实际引用需核实数据库)：
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1. **文献名称**：*RASSF8 regulates intercellular adhesion and actomyosin contractility via LIMK1*
**作者**：Vlahov, N., et al.
**摘要**：本研究利用RASSF8特异性抗体，通过免疫荧光和免疫印迹技术，揭示了RASSF8蛋白通过调控LIM激酶1(LIMK1)活性参与细胞间粘附和细胞骨架动态平衡的分子机制。
2. **文献名称**：*Expression and Clinical Significance of RASSF8 in Colorectal Cancer*
**作者**：Li, J., et al.
**摘要**：通过免疫组化技术(使用RASSF8多克隆抗体)，研究发现RASSF8在结直肠癌组织中表达显著下调，其低表达与患者预后不良相关，提示其可能作为肿瘤抑制因子发挥作用。
3. **文献名称**：*Proteomic Analysis of RASSF8-Interacting Proteins in Epithelial Cells*
**作者**：Kumar, R., et al.
**摘要**：利用RASSF8抗体进行免疫共沉淀(Co-IP)实验，鉴定了RASSF8在上皮细胞中的相互作用蛋白网络，表明其在细胞极性和信号转导中的潜在功能。
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**注**：以上文献信息为示例，实际研究中建议通过PubMed、Web of Science等数据库以“RASSF8 antibody”或“RASSF8 immunohistochemistry”等关键词检索最新文献，并优先选择经同行评审的期刊论文。
The RASSF8 antibody is a tool used to detect and study the Ras association domain-containing protein 8 (RASSF8), a member of the RASSF family implicated in tumor suppression and cellular adhesion. RASSF8. also known as HOJ-1. is a scaffolding protein involved in regulating cell-cell adhesion, cytoskeletal organization, and potentially cell proliferation or apoptosis. Structurally, it contains a Ras-associated (RA) domain and coiled-coil motifs, enabling interactions with signaling molecules and cytoskeletal components. RASSF8 is expressed in various tissues, with higher levels observed in brain, testis, and kidney. Dysregulation of RASSF8 has been linked to cancer progression, though its role remains less characterized compared to other RASSF members like RASSF1A.
The antibody aids in investigating RASSF8's localization (e.g., plasma membrane, adherens junctions) and expression patterns via techniques like Western blotting, immunohistochemistry, or immunofluorescence. Research suggests RASSF8 may modulate Hippo signaling, a pathway critical for tissue homeostasis and tumor suppression, and interact with adherens junction proteins (e.g., E-cadherin) to maintain epithelial integrity. Its potential tumor-suppressive function involves promoting apoptosis and inhibiting cell migration. However, conflicting studies indicate context-dependent roles, necessitating further validation. Commercial RASSF8 antibodies are typically validated for specificity in human or murine models, supporting ongoing studies to clarify its biological mechanisms and therapeutic relevance in diseases like cancer.
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