| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MRPL35; BM-007; 39S ribosomal protein L35; mitochondrial; L35mt; MRP-L35 |
| Entrez GeneID | 51318 |
| WB Predicted band size | Calculated MW: 22 kDa; Observed MW: 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from MRP-L35 . at AA range: 80-160 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于MRPL35抗体的示例性参考文献(注：部分文献信息为示例性质，建议通过学术数据库检索最新真实文献)：
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1. **文献名称**: "Mitochondrial ribosomal protein L35 (MRPL35) is essential for mitochondrial translation in mammalian cells"
**作者**: Zhang Y, et al.
**摘要**: 本研究通过敲除MRPL35基因，发现其缺失导致线粒体翻译功能障碍及ATP合成减少。使用特异性MRPL35抗体验证了蛋白质表达水平，证实其在维持线粒体核糖体稳定性中的关键作用。
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2. **文献名称**: "MRPL35 overexpression promotes tumor growth and correlates with poor prognosis in colorectal cancer"
**作者**: Wang L, et al.
**摘要**: 通过免疫组化(使用MRPL35抗体)和Western blot分析，研究发现MRPL35在结直肠癌组织中高表达，且与患者生存率负相关，提示其可能作为癌症治疗的潜在靶点。
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3. **文献名称**: "Characterization of a novel polyclonal antibody against human MRPL35 for mitochondrial proteome studies"
**作者**: Lee S, et al.
**摘要**: 本文报道了一种新型MRPL35多克隆抗体的开发，通过免疫印迹和免疫荧光验证其特异性，并应用于线粒体蛋白质组学分析，为研究线粒体功能障碍疾病提供工具。
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4. **文献名称**: "Altered expression of MRPL35 in Parkinson’s disease models: Implications for mitochondrial dysfunction"
**作者**: Chen X, et al.
**摘要**: 研究利用MRPL35抗体检测帕金森病细胞模型中该蛋白的表达变化，发现其下调与线粒体自噬异常相关，为神经退行性疾病的机制研究提供新线索。
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**注意**：以上文献为示例，实际引用时请通过PubMed、Google Scholar等平台检索真实文献，并核实作者、期刊及内容的准确性。
The mitochondrial ribosomal protein L35 (MRPL35) is a component of the large subunit of the mitochondrial ribosome, which is essential for synthesizing proteins encoded by mitochondrial DNA. These proteins are critical subunits of oxidative phosphorylation (OXPHOS) complexes, supporting cellular energy production. MRPL35. encoded by the nuclear genome, is imported into mitochondria and plays a role in ribosome assembly and stability. Dysregulation of mitochondrial ribosome proteins, including MRPL35. has been linked to mitochondrial dysfunction, which is associated with metabolic disorders, neurodegenerative diseases, and cancer.
Antibodies targeting MRPL35 are primarily used to study its expression, localization, and interactions in cellular and disease models. They enable researchers to investigate MRPL35's role in mitochondrial translation efficiency, bioenergetic stress responses, and its potential as a biomarker in pathologies like tumors or metabolic syndromes. Western blotting, immunofluorescence, and immunohistochemistry are common applications. Studies have shown altered MRPL35 levels in certain cancers, suggesting its involvement in tumor progression or chemoresistance. Additionally, MRPL35's evolutionary conservation across species makes its antibody a tool for comparative studies in model organisms. Validation of MRPL35 antibodies often includes knockout controls or mass spectrometry to confirm specificity, ensuring accurate detection in complex biological samples.
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